Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/100161
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Type: Journal article
Title: Intranasal vaccination with γ -irradiated Streptococcus pneumoniae whole-cell vaccine provides serotype-independent protection mediated by B-cells and innate IL-17 responses
Other Titles: Intranasal vaccination with gamma -irradiated Streptococcus pneumoniae whole-cell vaccine provides serotype-independent protection mediated by B-cells and innate IL-17 responses
Author: Babb, R.
Chen, A.
Hirst, T.
Kara, E.
McColl, S.
Ogunniyi, A.
Paton, J.
Alsharifi, M.
Citation: Clinical Science, 2016; 130(9):697-710
Publisher: Portland Press
Issue Date: 2016
ISSN: 0143-5221
1470-8736
Statement of
Responsibility: 
Rachelle Babb, Austen Chen, Timothy R. Hirst, Ervin E. Kara, Shaun R. McColl, Abiodun D. Ogunniyi, James C. Paton, and Mohammed Alsharifi
Abstract: Generating a pneumococcal vaccine that is serotype independent and cost effective remains a global challenge. γ-Irradiation has been used widely to sterilize biological products. It can also be utilized as an inactivation technique to generate whole-cell bacterial and viral vaccines with minimal impact on pathogen structure and antigenic determinants. In the present study, we utilized γ-irradiation to inactivate an un-encapsulated Streptococcus pneumoniae strain Rx1 with an unmarked deletion of the autolysin gene lytA and with the pneumolysin gene ply replaced with an allele encoding a non-toxic pneumolysoid (PdT) (designated γ-PN vaccine). Intranasal vaccination of C57BL/6 mice with γ-PN was shown to elicit serotype-independent protection in lethal challenge models of pneumococcal pneumonia and sepsis. Vaccine efficacy was shown to be reliant on B-cells and interleukin (IL)-17A responses. Interestingly, immunization promoted IL-17 production by innate cells not T helper 17 (Th17) cells. These data are the first to report the development of a non-adjuvanted intranasal γ-irradiated pneumococcal vaccine that generates effective serotype-independent protection, which is mediated by both humoral and innate IL-17 responses.
Keywords: Protective immunity; serotype independent; Streptococcus pneumoniae; vaccine
Rights: © 2016 Authors; published by Portland Press Limited
RMID: 0030042519
DOI: 10.1042/CS20150699
Grant ID: http://purl.org/au-research/grants/arc/LP120200244
Appears in Collections:Molecular and Biomedical Science publications

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