Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/100536
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Type: Journal article
Title: The spatial organization and extraction of the wall-forming bodies of Eimeria maxima
Author: Frölich, S.
Johnson, M.
Robinson, M.
Entzeroth, R.
Wallach, M.
Citation: Parasitology, 2013; 140(7):876-887
Publisher: Cambridge University Press
Issue Date: 2013
ISSN: 0031-1820
1469-8161
Statement of
Responsibility: 
Sonja Frölich, Michael Johnson, Michelle Robinson, Rolf Entzeroth, and Michael Wallach
Abstract: Eimeria maxima has been used as a model apicomplexan parasite to study sexual stage development and oocyst wall formation. A complete understanding of the wall's biochemical and biophysical properties is of great interest in research on all apicomplexan parasites. Purified gametocytes, zygotes and oocysts were analysed by three-dimensional confocal microscopy, and wide-field fluorescent microscopy was used to investigate the appearance and spatial organization of the 2 types of wall-forming bodies (WFBs). In addition, a variety of staining procedures and immunoassays were used to assess the biosynthesis, metabolic activity, intactness and molecular composition of the WFBs in situ. WFBs were extracted from gametocytes/zygotes and their composition was assessed by microscopy and SDS-PAGE analysis. It was concluded that isolated gametocytes are intact and metabolically active. Additionally, it was observed that the Type 1 WFBs are aligned at the periphery of the parasite and fuse together producing neutral lipid rich patches that appear to be inserted into the space between 2 parasite-specific membranes. Finally, it was shown that the WFBs extracted from purified gametocytes had the same shape, size and staining properties as those observed in situ, and contained the major glycoprotein antigens known to be present in these organelles.
Keywords: Eimeria maxima; gametocytes; wall-forming bodies; three-dimensional confocal microscopy; lipid patches; oocyst wall
Rights: © Cambridge University Press 2013
RMID: 0030045924
DOI: 10.1017/S0031182012002247
Appears in Collections:Medical Sciences publications

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