Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/101241
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Type: Journal article
Title: Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis
Author: Bi, H.
Luang, S.
Li, Y.
Bazanova, N.
Morran, S.
Song, Z.
Perera, M.
Hrmova, M.
Borisjuk, N.
Lopato, S.
Citation: Journal of Experimental Botany, 2016; 67(18):5363-5380
Publisher: Oxford University Press
Issue Date: 2016
ISSN: 1460-2431
1460-2431
Statement of
Responsibility: 
Huihui Bi, Sukanya Luang, Yuan Li, Natalia Bazanova, Sarah Morran, Zhihong Song, M. Ann Perera, Maria Hrmova, Nikolai Borisjuk, and Sergiy Lopato
Abstract: A plant cuticle forms a hydrophobic layer covering plant organs, and plays an important role in plant development and protection from environmental stresses. We examined epicuticular structure, composition, and a MYB-based regulatory network in two Australian wheat cultivars, RAC875 and Kukri, with contrasting cuticle appearance (glaucousness) and drought tolerance. Metabolomics and microscopic analyses of epicuticular waxes revealed that the content of β-diketones was the major compositional and structural difference between RAC875 and Kukri. The content of β-diketones remained the same while those of alkanes and primary alcohols were increased by drought in both cultivars, suggesting that the interplay of all components rather than a single one defines the difference in drought tolerance between cultivars. Six wheat genes encoding MYB transcription factors (TFs) were cloned; four of them were regulated in flag leaves of both cultivars by rapid dehydration and/or slowly developing cyclic drought. The involvement of selected MYB TFs in the regulation of cuticle biosynthesis was confirmed by a transient expression assay in wheat cell culture, using the promoters of wheat genes encoding cuticle biosynthesis-related enzymes and the SHINE1 (SHN1) TF. Two functional MYB-responsive elements, specifically recognized by TaMYB74 but not by other MYB TFs, were localized in the TdSHN1 promoter. Protein structural determinants underlying the binding specificity of TaMYB74 for functional DNA cis-elements were defined, using 3D protein molecular modelling. A scheme, linking drought-induced expression of the investigated TFs with downstream genes that participate in the synthesis of cuticle components, is proposed.
Keywords: Abiotic stress; MYB and SHINE1 transcription factors; cuticle; drought; molecular model; water deficit; wax; wheat.; β-diketone
Rights: © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030051796
DOI: 10.1093/jxb/erw298
Appears in Collections:Agriculture, Food and Wine publications

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