Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||A rapid wire-based sampling method for DNA profiling*|
|Citation:||Journal of Forensic Sciences, 2012; 57(2):472-477|
|Tong Chen, David E. A. Catcheside, Alice Stephenson, Chris Hefford, K. Paul Kirkbride, and Leigh A. Burgoyne|
|Abstract:||This paper reports the results of a commission to develop a field deployable rapid short tandem repeat (STR)-based DNA profiling system to enable discrimination between tissues derived from a small number of individuals. Speed was achieved by truncation of sample preparation and field deployability by use of an Agilent 2100 Bioanalyser™. Human blood and tissues were stabbed with heated stainless steel wire and the resulting sample dehydrated with isopropanol prior to direct addition to a PCR. Choice of a polymerase tolerant of tissue residues and cycles of amplification appropriate for the amount of template expected yielded useful profiles with a custom-designed quintuplex primer set suitable for use with the Bioanalyser™. Samples stored on wires remained amplifiable for months, allowing their transportation unrefrigerated from remote locations to a laboratory for analysis using AmpFlSTR® Profiler Plus® without further processing. The field system meets the requirements for discrimination of samples from small sets and retains access to full STR profiling when required.|
|Keywords:||forensic science; biological sampling; DNA profiling; stainless steel wire; disaster victim identification|
|Description:||First published: 28 December 2011|
|Rights:||© 2011 American Academy of Forensic Sciences|
|Appears in Collections:||Animal and Veterinary Sciences publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.