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https://hdl.handle.net/2440/102144
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DC Field | Value | Language |
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dc.contributor.author | Westley, I. | - |
dc.contributor.author | Coller, J. | - |
dc.contributor.author | Ward, M. | - |
dc.contributor.author | Evans, A. | - |
dc.contributor.author | Morris, R. | - |
dc.contributor.author | Sallustio, B. | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Journal of Liquid Chromatography and Related Technologies, 2014; 37(9):1249-1256 | - |
dc.identifier.issn | 1082-6076 | - |
dc.identifier.issn | 1520-572X | - |
dc.identifier.uri | http://hdl.handle.net/2440/102144 | - |
dc.description.abstract | Background: A number of single nucleotide polymorphisms have been described in the ABCC2 gene that alters drug disposition. The aim of the study was to develop a primer extension denaturing high-performance liquid chromatography (PE-dHPLC) assay to determine three common variants of the ABCC2 gene in the promoter region (−24C>T), exon 10 (1249G>A), and exon 28 (3972C>T). Methods: Polymerase chain reactions (PCRs) were used to isolate the area of interest in the ABCC2 gene. A comparison of the PCR product was performed between sequencing and dHPLC. Results: A 100% identity match was achieved between groups and allowed for a quick and accurate method to determine three single nucleotide polymorphisms in a single extension reaction. This assay is the first of its type to determine three ABCC2 variants by dHPLC | - |
dc.description.statementofresponsibility | Ian S. Westley, Janet K. Coller, Michael B. Ward, Allan M. Evans, Raymond G. Morris, and Benedetta C. Sallustio | - |
dc.language.iso | en | - |
dc.publisher | Taylor and Francis | - |
dc.rights | Copyright © Taylor & Francis Group, LLC | - |
dc.source.uri | http://dx.doi.org/10.1080/10826076.2013.789796 | - |
dc.subject | ABCC2; genotype; polymerase chain reaction; polymorphisms; PE-dHPLC; single nudeotide polymorphism | - |
dc.title | A primer extension denaturing high-performance liquid chromatography method for the identification of three ABCC2 genetic polymorphisms | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1080/10826076.2013.789796 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Coller, J. [0000-0002-8273-5048] | - |
dc.identifier.orcid | Sallustio, B. [0000-0002-0186-3073] | - |
Appears in Collections: | Aurora harvest 7 Medical Sciences publications |
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