Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/102783
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Type: Journal article
Title: Expression and distribution of peroxiredoxins in the retina and optic nerve
Author: Chidlow, G.
Wood, J.
Knoops, B.
Casson, R.
Citation: Brain Structure and Function, 2016; 221(8):3903-3925
Publisher: Springer-Verlag
Issue Date: 2016
ISSN: 1863-2653
1863-2661
Statement of
Responsibility: 
Glyn Chidlow, John P. M. Wood, Bernard Knoops, Robert J. Casson
Abstract: Oxidative stress is implicated in various pathological conditions of the retina and optic nerve. Peroxiredoxins (Prdxs) comprise a recently characterized family of antioxidant enzymes. To date, little information exists regarding the distribution of Prdxs in the eye. Herein, we employed a combination of qRT-PCR, immunohistochemistry and Western blotting to determine the level of expression and distribution of the six Prdx isoforms in the retina and optic nerve of the rat. In addition, we performed some parallel analyses on the common marmoset (Callithrix Jacchus). In the rat, all of the Prdx transcripts were expressed in relatively high amounts in both retina and optic nerve, with abundances ranging from approximately 3-50 % of the level of the housekeeping gene cyclophilin. With regard to protein expression, each isoform was detected in the retina and optic nerve by either Western blotting and/or immunohistochemistry. Excepting Prdx4, there was a good correspondence between the rodent and primate results. In the retina, Prdx1 and Prdx2 were principally localized to neurons in the inner nuclear layer and cone photoreceptors, Prdx3 and Prdx5 displayed characteristic mitochondrial immunolabeling, while Prdx6 was associated with astrocytes and Müller cells. In the optic nerve, Prdx1 was robustly expressed by oligodendrocytes, Prdx3 and Prdx5 were observed in axons, and Prdx6 was restricted to astrocytes. The present findings augment our understanding of the distribution and expression of the Prdxs in the retina and optic nerve of rodents and primates and lay the foundation for subsequent analysis of their involvement in relevant blinding diseases.
Keywords: Antioxidant enzyme; Immunohistochemistry; RT-PCR; Western blotting; Glia; Neurons
Description: Published online: 26 October 2015
Rights: © The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://crea tivecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
RMID: 0030038381
DOI: 10.1007/s00429-015-1135-3
Grant ID: http://purl.org/au-research/grants/nhmrc/1050982
Appears in Collections:Opthalmology & Visual Sciences publications

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