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Type: Journal article
Title: Maize maintains growth in response to decreased nitrate supply through a highly dynamic and developmental stage-specific transcriptional response
Author: Plett, D.
Baumann, U.
Schreiber, A.
Holtham, L.
Kalashyan, E.
Toubia, J.
Nau, J.
Beatty, M.
Rafalski, A.
Dhugga, K.
Tester, M.
Garnett, T.
Kaiser, B.
Citation: Plant Biotechnology Journal, 2016; 14(1):342-353
Publisher: Wiley
Issue Date: 2016
ISSN: 1467-7644
Statement of
Darren Plett, Ute Baumann, Andreas W. Schreiber, Luke Holtham, Elena Kalashyan, John Toubia, John Nau, Mary Beatty, Antoni Rafalski, Kanwarpal S. Dhugga, Mark Tester, Trevor Garnett and Brent N. Kaiser
Abstract: Elucidation of the gene networks underlying the response to N supply and demand will facilitate the improvement of the N uptake efficiency of plants. We undertook a transcriptomic analysis of maize to identify genes responding to both a non-growth-limiting decrease in NO3- provision and to development-based N demand changes at seven representative points across the life cycle. Gene co-expression networks were derived by cluster analysis of the transcript profiles. The majority of NO3--responsive transcription occurred at 11 (D11), 18 (D18) and 29 (D29) days after emergence, with differential expression predominating in the root at D11 and D29 and in the leaf at D18. A cluster of 98 probe sets was identified, the expression pattern of which is similar to that of the high-affinity NO3- transporter (NRT2) genes across the life cycle. The cluster is enriched with genes encoding enzymes and proteins of lipid metabolism and transport, respectively. These are candidate genes for the response of maize to N supply and demand. Only a few patterns of differential gene expression were observed over the entire life cycle; however, the composition of the classes of the genes differentially regulated at individual time points was unique, suggesting tightly controlled regulation of NO3--responsive gene expression.
Keywords: N use efficiency; high-affinity nitrate transporter; NRT2; microarray; gene cluster analysis; lipid metabolism
Rights: © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd
RMID: 0030030043
DOI: 10.1111/pbi.12388
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Appears in Collections:Agriculture, Food and Wine publications

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