Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/109089
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Type: Journal article
Title: The pneumococcal alpha-glycerophosphate oxidase enhances nasopharyngeal colonization through binding to host glycoconjugates
Author: Mahdi, L.
Higgins, M.
Day, C.
Tiralongo, J.
Hartley-Tassell, L.
Jennings, M.
Gordon, D.
Paton, A.
Paton, J.
Ogunniyi, A.
Citation: EBioMedicine, 2017; 18:236-243
Publisher: Elsevier
Issue Date: 2017
ISSN: 2352-3964
2352-3964
Statement of
Responsibility: 
Layla K. Mahdi, Melanie A. Higgins, Christopher J. Day, Joe Tiralongo, Lauren E. Hartley-Tassell, Michael P. Jennings, David L. Gordon, Adrienne W. Paton, James C. Paton, Abiodun D. Ogunniyi
Abstract: Streptococcus pneumoniae (the pneumococcus) is a major human pathogen, causing a broad spectrum of diseases including otitis media, pneumonia, bacteraemia and meningitis. Here we examined the role of a potential pneumococcal meningitis vaccine antigen, alpha-glycerophosphate oxidase (SpGlpO), in nasopharyngeal colonization. We found that serotype 4 and serotype 6A strains deficient in SpGlpO have significantly reduced capacity to colonize the nasopharynx of mice, and were significantly defective in adherence to human nasopharyngeal carcinoma cells in vitro. We also demonstrate that intranasal immunization with recombinant SpGlpO significantly protects mice against subsequent nasal colonization by wild type serotype 4 and serotype 6A strains. Furthermore, we show that SpGlpO binds strongly to lacto/neolacto/ganglio host glycan structures containing the GlcNAcβ1-3Galβ disaccharide, suggesting that SpGlpO enhances colonization of the nasopharynx through its binding to host glycoconjugates. We propose that SpGlpO is a promising vaccine candidate against pneumococcal carriage, and warrants inclusion in a multi-component protein vaccine formulation that can provide robust, serotype-independent protection against all forms of pneumococcal disease.
Keywords: Bacterial pathogens; Streptococcus pneumonia; protein vaccines; Pneumococcal disease; colonization; adherence; host glycoconjugates; alpha-glycerophosphate oxidase; immunization
Rights: © 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
RMID: 0030066934
DOI: 10.1016/j.ebiom.2017.03.002
Grant ID: http://purl.org/au-research/grants/nhmrc/565526
http://purl.org/au-research/grants/nhmrc/1071659
http://purl.org/au-research/grants/nhmrc/627142
Appears in Collections:Molecular and Biomedical Science publications

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