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https://hdl.handle.net/2440/110970
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Type: | Journal article |
Title: | Salinity effects on chloroplast PSII performance in glycophytes and halophytes |
Author: | Percey, W. McMinn, A. Bose, J. Breadmore, M. Guijt, R. Shabala, S. |
Citation: | Functional Plant Biology: an international journal of plant function, 2016; 43(11):1003-1015 |
Publisher: | CSIRO Publishing |
Issue Date: | 2016 |
ISSN: | 1445-4408 1445-4416 |
Statement of Responsibility: | William J. Percey, Andrew McMinn, Jayakumar Bose, Michael C. Breadmore, Rosanne M. Guijt and Sergey Shabala |
Abstract: | The effects of NaCl stress and K⁺ nutrition on photosynthetic parameters of isolated chloroplasts were investigated using PAM fluorescence. Intact mesophyll cells were able to maintain optimal photosynthetic performance when exposed to salinity for more than 24 h whereas isolated chloroplasts showed declines in both the relative electron transport rate (rETR) and the maximal photochemical efficiency of PSII (Fv/Fm) within the first hour of treatment. The rETR was much more sensitive to salt stress compared with Fv/Fm, with 40% inhibition of rETR observed at apoplastic NaCl concentration as low as 20 mM. In isolated chloroplasts, absolute K⁺ concentrations were more essential for the maintenance of the optimal photochemical performance (Fv/Fm values) rather than sodium concentrations per se. Chloroplasts from halophyte species of quinoa (Chenopodium quinoa Willd.) and pigface (Carpobrotus rosii (Haw.) Schwantes) showed less than 18% decline in Fv/Fm under salinity, whereas the Fv/Fm decline in chloroplasts from glycophyte pea (Pisum sativum L.) and bean (Vicia faba L.) species was much stronger (31 and 47% respectively). Vanadate (a P-type ATPase inhibitor) significantly reduced Fv/Fm in both control and salinity treated chloroplasts (by 7 and 25% respectively), whereas no significant effects of gadolinium (blocker of non-selective cation channels) were observed in salt-treated chloroplasts. Tetraethyl ammonium (TEA) (K⁺ channel inhibitor) and amiloride (inhibitor of the Na⁺/H⁺ antiporter) increased the Fv/Fm of salinity treated chloroplasts by 16 and 17% respectively. These results suggest that chloroplasts’ ability to regulate ion transport across the envelope and thylakoid membranes play a critical role in leaf photosynthetic performance under salinity. |
Keywords: | Membrane transport; non-stomatal limitation; photosynthesis; potassium; ROS; sodium |
Rights: | Journal compilation © CSIRO 2016 |
DOI: | 10.1071/FP16135 |
Grant ID: | http://purl.org/au-research/grants/arc/DP0987402 http://purl.org/au-research/grants/arc/LP100200456 |
Published version: | http://dx.doi.org/10.1071/fp16135 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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