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https://hdl.handle.net/2440/110971
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Type: | Journal article |
Title: | X-ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants |
Author: | van der Ent, A. Przybyłowicz, W.J. de Jonge, M.D. Harris, H.H. Ryan, C.G. Tylko, G. Paterson, D.J. Barnabas, A.D. Kopittke, P.M. Mesjasz-Przybyłowicz, J. |
Citation: | New Phytologist, 2017; 218(2):432-452 |
Publisher: | University of Queensland |
Issue Date: | 2017 |
ISSN: | 0028-646X 1469-8137 |
Statement of Responsibility: | Antony van der Ent, Wojciech J. Przybyłowicz, Martin D. de Jonge, Hugh H. Harris, Chris G. Ryan, Grzegorz Tylko, David J. Paterson, Alban D. Barnabas, Peter M. Kopittke and Jolanta Mesjasz-Przybyłowicz |
Abstract: | SUMMARY: Hyperaccumulators are attractive models for studying metal(loid) homeostasis, and probing the spatial distribution and coordination chemistry of metal(loid)s in their tissues is important for advancing our understanding of their ecophysiology. X-ray elemental mapping techniques are unique in providing in situ information, and with appropriate sample preparation offer results true to biological conditions of the living plant. The common platform of these techniques is a reliance on characteristic X-rays of elements present in a sample, excited either by electrons (scanning/transmission electron microscopy), protons (proton-induced X-ray emission) or X-rays (X-ray fluorescence microscopy). Elucidating the cellular and tissue-level distribution of metal(loid)s is inherently challenging and accurate X-ray analysis places strict demands on sample collection, preparation and analytical conditions, to avoid elemental redistribution, chemical modification or ultrastructural alterations. We compare the merits and limitations of the individual techniques, and focus on the optimal field of applications for inferring ecophysiological processes in hyperaccumulator plants. X-ray elemental mapping techniques can play a key role in answering questions at every level of metal(loid) homeostasis in plants, from the rhizosphere interface, to uptake pathways in the roots and shoots. Further improvements in technological capabilities offer exciting perspectives for the study of hyperaccumulator plants into the future. |
Keywords: | cryo-fixation freeze-drying frozen-hydrated state micro-PIXE nuclear/proton microprobe scanning electron microscopy synchrotron X-ray absorption spectroscopy synchrotron X-ray fluorescence microscopy (XFM) |
Rights: | © 2017 University of Queensland New Phytologist © 2017 New Phytologist Trust |
DOI: | 10.1111/nph.14810 |
Grant ID: | ARC http://purl.org/au-research/grants/arc/FT120100277 http://purl.org/au-research/grants/arc/DP140100176 |
Published version: | http://dx.doi.org/10.1111/nph.14810 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 8 |
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