Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/112013
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Type: Journal article
Title: The wheat TabZIP2 transcription factor is activated by the nutrient starvation-responsive SnRK3/CIPK protein kinase
Author: Luang, S.
Sornaraj, P.
Bazanova, N.
Jia, W.
Eini, O.
Hussain, S.
Kovalchuk, N.
Agarwal, P.
Hrmova, M.
Lopato, S.
Citation: Plant Molecular Biology, 2018; 96(6):543-561
Publisher: Kluwer Academic Publishers
Issue Date: 2018
ISSN: 0167-4412
1573-5028
Statement of
Responsibility: 
Sukanya Luang, Pradeep Sornaraj, Natalia Bazanova, Wei Jia, Omid Eini, Syed Sarfraz Hussain, Nataliya Kovalchuk, Pradeep K. Agarwal, Maria Hrmova, Sergiy Lopato
Abstract: Basic leucine zipper (bZIP) transcription factors (TFs) play key roles in the regulation of grain development and plant responses to abiotic stresses. We investigated the role and molecular mechanisms of function of the TabZIP2 gene isolated from drought-stressed wheat plants. Molecular characterisation of TabZIP2 and derived protein included analyses of gene expression and its target promoter, and the influence of interacting partners on the target promoter activation. Two interacting partners of TabZIP2, the 14-3-3 protein, TaWIN1 and the bZIP transcription factor TaABI5L, were identified in a Y2H screen. We established that under elevated ABA levels the activity of TabZIP2 was negatively regulated by the TaWIN1 protein and positively regulated by the SnRK3/CIPK protein kinase WPK4, reported previously to be responsive to nutrient starvation. The physical interaction between the TaWIN1 and the WPK4 was detected. We also compared the influence of homo- and hetero-dimerisation of TabZIP2 and TaABI5L on DNA binding. TabZIP2 gene functional analyses were performed using drought-inducible overexpression of TabZIP2 in transgenic wheat. Transgenic plants grown under moderate drought during flowering, were smaller than control plants, and had fewer spikes and seeds per plant. However, a single seed weight was increased compared to single seed weights of control plants in three of four evaluated transgenic lines. The observed phenotypes of transgenic plants and the regulation of TabZIP2 activity by nutrient starvation-responsive WPK4, suggest that the TabZIP2 could be the part of a signalling pathway, which controls the rearrangement of carbohydrate and nutrient flows in plant organs in response to drought.
Keywords: 14-3-3 protein; 3D protein molecular modelling; drought; homo- and hetero-dimerisation; SnRK3/CIPK protein kinase; wheat
Rights: © Springer Science+Business Media B.V., part of Springer Nature 2018
RMID: 0030084329
DOI: 10.1007/s11103-018-0713-1
Grant ID: http://purl.org/au-research/grants/arc/LP120100201
Appears in Collections:Agriculture, Food and Wine publications

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