Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/112127
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Type: Journal article
Title: A mini-multiplex SNaPshot assay for the triage of degraded human DNA
Author: Bardan, F.
Higgins, D.
Austin, J.
Citation: Forensic Science International: Genetics, 2018; 34:62-70
Publisher: Elsevier BV
Issue Date: 2018
ISSN: 1872-4973
1878-0326
Statement of
Responsibility: 
Felicia Bardan, Denice Higgins, Jeremy J. Austin
Abstract: Short Tandem Repeat (STR) genotyping is currently the primary DNA-based method for human identification, however it can have limited success when applied to degraded human remains. Massively parallel sequencing (MPS) provides new opportunities to obtain genetic data for hundreds of loci in a single assay with higher success from degraded samples. However, due to the extra requirement for specialised equipment, expertise and resources, routine use of MPS may not be feasible or necessary for many forensic cases. Here we describe the development of a mini-multiplex SNaPshot screening tool (Miniplex) for human samples which allows the qualitative comparison of short mitochondrial and nuclear DNA targets, as well as the interrogation of biogeographic ancestry, lineage, and phenotype single nucleotide polymorphisms (SNPs). This tool is useful to triage samples based on sample quality prior to downstream identification workflows and provides broad biological profile data for intelligence purposes.
Keywords: Degraded DNA; Screening tool; SNPs; Biogeographic ancestry; Sex prediction; Lineage markers
Description: Available online 06 February 2018
Rights: © 2018 Elsevier B.V. All rights reserved.
RMID: 0030081850
DOI: 10.1016/j.fsigen.2018.02.006
Grant ID: http://purl.org/au-research/grants/arc/FT100100108
http://purl.org/au-research/grants/arc/DP150101664
http://purl.org/au-research/grants/arc/LE160100154
Appears in Collections:Australian Centre for Ancient DNA publications

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