Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/112607
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Type: Journal article
Title: Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation
Author: Schlötterer, C.
Kofler, R.
Versace, E.
Tobler, R.
Franssen, S.
Citation: Heredity, 2015; 114(5):431-440
Publisher: Springer Nature
Issue Date: 2015
ISSN: 0018-067X
1365-2540
Statement of
Responsibility: 
C Schlötterer, R Kofler, E Versace, R Tobler and SU Franssen
Abstract: Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype–phenotype link and serve as an important research tool for a broad range of evolutionary questions.
Keywords: Animals; Drosophila melanogaster; Adaptation, Physiological; Gene Frequency; Genotype; Phenotype; Polymorphism, Single Nucleotide; Research Design; Genetic Variation; Genetic Association Studies; Biological Evolution; High-Throughput Nucleotide Sequencing
Rights: © 2015 Macmillan Publishers Limited All rights reserved This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
RMID: 0030066494
DOI: 10.1038/hdy.2014.86
Appears in Collections:Genetics publications

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