Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/11265
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dc.contributor.authorJitrapakdee, S.-
dc.contributor.authorWalker, M.-
dc.contributor.authorWallace, J.-
dc.date.issued1996-
dc.identifier.citationBiochemical and Biophysical Research Communications, 1996; 223(3):695-700-
dc.identifier.issn0006-291X-
dc.identifier.issn1090-2104-
dc.identifier.urihttp://hdl.handle.net/2440/11265-
dc.description.abstractWe have identified and characterized multiple mRNA transcripts of rat and human pyruvate carboxylases [EC 6.4.1.1] using rapid amplification of cDNA 5' ends-polymerase chain reaction (RACE-PCR). Five alternative forms of rat pyruvate carboxylase cDNAs have been identified in liver, kidney, brain, and adipose tissue and these are expressed in a tissue-specific manner. Two alternative forms of human pyruvate carboxylase cDNA have also been identified in liver. These pyruvate carboxylase cDNAs have a common coding region but differ in their 5' untranslated regions (5'UTRs), suggesting that they are generated by alternative splicing of the primary transcript. Southern blot analysis of restriction enzyme digested rat genomic DNA revealed that pyruvate carboxylase is encoded by a single copy gene.-
dc.language.isoen-
dc.publisherACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS-
dc.source.urihttp://dx.doi.org/10.1006/bbrc.1996.0958-
dc.subjectKidney-
dc.subjectAnimals-
dc.subjectHumans-
dc.subjectRats-
dc.subjectIsoenzymes-
dc.subjectPyruvate Carboxylase-
dc.subjectRecombinant Proteins-
dc.subjectRNA, Messenger-
dc.subjectDNA Primers-
dc.subjectCloning, Molecular-
dc.subjectRandom Amplified Polymorphic DNA Technique-
dc.subjectProtein Biosynthesis-
dc.subjectAlternative Splicing-
dc.subjectBase Sequence-
dc.subjectRegulatory Sequences, Nucleic Acid-
dc.subjectSequence Homology, Nucleic Acid-
dc.subjectMolecular Sequence Data-
dc.titleIdentification of Novel Alternatively Spliced Pyruvate Carboxylase mRNAs with Divergent 5' -Untranslated Regions Which Are Expressed in a Tissue-Specific Manner-
dc.typeJournal article-
dc.identifier.doi10.1006/bbrc.1996.0958-
pubs.publication-statusPublished-
dc.identifier.orcidWalker, M. [0000-0002-6934-3787]-
Appears in Collections:Aurora harvest 2
Biochemistry publications

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