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|Title:||Polymorphism of the yeast pyruvate carboxylase 2 gene and protein: Effects on biotinylation|
|Citation:||Biochemical Journal, 1995; 312(3):817-825|
|Publisher:||The Biochemical Society, London|
|Abstract:||In Saccharomyces cerevisiae there are two isoenzymes of pyruvate carboxylase (Pyc) encoded by separate genes designated PYC1 and PYC2. We report the isolation and sequencing of a PYC2 gene, and the localization of both genes on the physical map of S. cerevisiae. Comparison with the previously reported sequence [Stucka, Dequin, Salmon and Gancedo (1991) Mol. Gen. Genet. 229, 307-315] revealed significant differences within the open reading frame. The most notable difference was near the 3' end, where we found a single base deletion reducing the open reading frame by 15 bases. We have confirmed the C-terminus of Pyc2 encoded by the gene isolated here by expressing and purifying an 86-amino-acid biotin-domain peptide. In addition, we investigated the effects of the two changes in the Pyc2 biotin domain (K1155R substitution and Q1178P/five-amino-acid extension) on the extent of biotinylation in vivo by Escherichia coli biotin ligase, and compared the biotinylation of peptides containing these changes with that of two different-length Pyc1 biotin-domain peptides. The K1155R substitution had very little effect on biotinylation, but the five-amino-acid C-terminal extension to Pyc2 and the N-terminal extension to Pycl both improved biotinylation in vivo.|
|Keywords:||Escherichia coli; Saccharomyces cerevisiae; Biotin; Isoenzymes; Pyruvate Carboxylase; DNA, Fungal; Chromosome Mapping; Sequence Analysis; Mutagenesis; Binding Sites; Amino Acid Sequence; Base Sequence; Sequence Homology; Polymorphism, Genetic; Genes, Fungal; Molecular Sequence Data|
|Appears in Collections:||Biochemistry publications|
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