Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/112722
Type: | Theses |
Title: | Intergenomic SNPs reveal putative spontaneous chromosomal interchanges between chromosomes 7A and 7D of wheat |
Author: | Nguyen, Phuoc Xuan |
Issue Date: | 2016 |
School/Discipline: | School of Agriculture, Food and Wine |
Abstract: | Langdon 7D(7A) and 7D(7B) durum substitution lines were crossed with DBA-Aurora durum wheat to introgress a lutein esterification gene,TaGelp1, from chromosome 7D onto its homoeologues 7A and 7B. Genotyping-by-sequencing based on DNA samples from durum wheat and bread wheat revealed single nucleotide polymorphism (SNPs) among the group-7 chromosomes. Sixteen KASP markers were developed and to be able to differentiate among these chromosomes. Nine 7A-7D markers were used to characterise progeny populations to search for dissociation of molecular markers which may indicate chromosomal recombination. Evidence of possible 7A-7D recombination was found in a small number of progeny (less than 4%). Most of the putative marker dissociations were in the centromeric region but one plant was found to carry only a small distal fragment of 7DS including TaGelp1. The findings suggest crossing normal durum with Langdon 7D(7A) combined with KASP marker assistance can be applied as a method to introgress and assess genes from chromosome 7D onto its homoeologues without resorting to use of wheat with the Ph1 deletion. |
Advisor: | Mather, Diane Elizabeth |
Dissertation Note: | Thesis (M.Bio.(PB)) -- University of Adelaide, Masters of Biotechnology (Plant Biotechnology), School of Agriculture, Food and Wine, 2016. |
Keywords: | coursework Triticum turgidum subsp. durum lutein esterification genotyping-by-sequencing KASP marker homoeologous recombination |
Description: | Title page, abstract and table of contents only. The complete thesis in print form is available from the University of Adelaide Library. |
Provenance: | Masters of Biotechnology (Plant Biotechnology) by coursework |
Appears in Collections: | School of Agriculture, Food and Wine |
Files in This Item:
File | Description | Size | Format | |
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09jmn5764.pdf | Title page, abstract and table of contents only | 70.79 kB | Adobe PDF | View/Open |
Restricted Restricted Access | Archival - Library staff access only | 483.41 kB | Adobe PDF | View/Open |
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