Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/11279
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Type: Journal article
Title: Splice variants of the mouse Tec gene are differentially expressed in vivo
Author: Merkel, A.
Atmosukarto, I.
Stevens, K.
Rathjen, P.
Booker, G.
Citation: Cytogenetics and Cell Genetics, 1999; 84(1-2):132-139
Publisher: KARGER
Issue Date: 1999
ISSN: 0301-0171
1424-859X
Abstract: Tec is a cytoplasmic protein tyrosine kinase that participates in the signalling pathways of a broad range of cytokines. Up to five different Tec isoforms have been reported in the literature. We report here the genomic organisation of the mouse Tec gene and the tissue expression pattern of the two predominant transcripts, TecIII and TecIV. The mouse Tec gene consists of 18 exons, spans more than 86 kb, and is 2.6 kb 5' to the gene for Txk, a Tec family member. Comparison of mouse and human Btk, human TXK, and mouse Tec genomic structures shows a high level of conservation of exon/intron boundaries. Compared with TecIV, the TecIII transcript has a 66-bp deletion in the SH3 domain encoding region and is revealed here to arise by alternative splicing of exon 8. We show that both TecIII and TecIV are expressed as early as embryonic day 10.5 in mouse development, as well as in adult and embryonic organs. The ratio of TecIV to TecIII expression is markedly reduced in adult liver and kidney tissues and d16 embryonic limb.
Keywords: Cell Line; Animals; Humans; Mice; RNA, Messenger; DNA Primers; Gene Expression; Alternative Splicing; Amino Acid Sequence; Base Sequence; Conserved Sequence; Sequence Homology, Amino Acid; Tissue Distribution; Introns; Exons; Molecular Sequence Data; Protein-Tyrosine Kinases
RMID: 0030004414
DOI: 10.1159/000015240
Appears in Collections:Biochemistry publications

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