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https://hdl.handle.net/2440/112908
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dc.contributor.author | Ramesh, S. | - |
dc.contributor.author | Kamran, M. | - |
dc.contributor.author | Sullivan, W. | - |
dc.contributor.author | Chirkova, L. | - |
dc.contributor.author | Okamoto, M. | - |
dc.contributor.author | Degryse, F. | - |
dc.contributor.author | McLauchlin, M. | - |
dc.contributor.author | Gilliham, M. | - |
dc.contributor.author | Tyerman, S. | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | The Plant Cell, 2018; 30(5):1147-1164 | - |
dc.identifier.issn | 1040-4651 | - |
dc.identifier.issn | 1532-298X | - |
dc.identifier.uri | http://hdl.handle.net/2440/112908 | - |
dc.description | Published April 4, 2018 | - |
dc.description.abstract | Plant aluminium-activated malate transporters (ALMTs) are currently classified as anion channels; they are also known to be regulated by diverse signals, leading to a range of physiological responses. Gamma-aminobutyric acid (GABA) regulation of anion flux through ALMT proteins requires a specific amino acid motif in ALMTs that shares similarity with a GABA-binding site in mammalian GABAA receptors. Here, we explore why TaALMT1-activation leads to a negative correlation between malate efflux and endogenous GABA concentrations ([GABA]i) in both wheat (Titicum aestivum L) root tips and in heterologous expression systems. We show that TaALMT1 activation reduces [GABA]i because TaALMT1 facilitates GABA efflux but GABA does not complex Al³⁺. TaALMT1 also leads to GABA transport into cells, demonstrated by a yeast complementation assay and via ¹⁴CGABA uptake into TaALMT1-expressing Xenopus laevis oocytes; this was found to be a general feature of all ALMTs we examined. Mutation of the GABA 'motif' (TaALMT1F²¹³C) prevented both GABA influx and efflux, and resulted in no correlation between malate efflux and [GABA]i. We conclude that ALMTs are likely to act as both GABA and anion transporters in planta. GABA and malate appear to interact with ALMTs in a complex manner to regulate each other's transport, suggestive of a role for ALMTs in communicating metabolic status. | - |
dc.description.statementofresponsibility | Sunita A. Ramesh, Muhammad Kamran, Wendy Sullivan, Larissa Chirkova, Mamoru Okamoto, Fien Degryse, Michael McLaughlin, Matthew Gilliham, and Stephen D. Tyerman | - |
dc.language.iso | en | - |
dc.publisher | American Society of Plant Biologists | - |
dc.rights | © 2018 ASPB. OPEN | - |
dc.source.uri | http://dx.doi.org/10.1105/tpc.17.00864 | - |
dc.subject | Triticum | - |
dc.subject | Aluminum | - |
dc.subject | gamma-Aminobutyric Acid | - |
dc.subject | Malates | - |
dc.subject | Plant Proteins | - |
dc.subject | Ion Transport | - |
dc.subject | Biological Transport | - |
dc.title | Aluminium-activated malate transporters can facilitate GABA transport | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1105/tpc.17.00864 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/CE140100008 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/IH130200027 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DP130104205 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/FT130100709 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Ramesh, S. [0000-0003-2230-4737] | - |
dc.identifier.orcid | Okamoto, M. [0000-0002-2989-607X] | - |
dc.identifier.orcid | Degryse, F. [0000-0002-4875-2944] | - |
dc.identifier.orcid | Gilliham, M. [0000-0003-0666-3078] | - |
dc.identifier.orcid | Tyerman, S. [0000-0003-2455-1643] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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