Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/113015
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Type: Journal article
Title: A novel model for acute peripheral nerve injury in the horse and evaluation of the effect of mesenchymal stromal cells applied in situ on nerve regeneration: a preliminary study
Author: Cruz Villagrán, C.
Schumacher, J.
Donnell, R.
Dhar, M.
Citation: Frontiers in Veterinary Science, 2016; 3:80
Publisher: Frontiers
Issue Date: 2016
ISSN: 2297-1769
2297-1769
Statement of
Responsibility: 
Claudia Cruz Villagrán, Jim Schumacher, Robert Donnell and Madhu S. Dhar
Abstract: Transplantation of mesenchymal stromal cells (MSCs) to sites of experimentally created nerve injury in laboratory animals has shown promising results in restoring nerve function. This approach for nerve regeneration has not been reported in horses. In this study, we first evaluated the in vitro ability of equine bone marrow-derived MSCs (EBM-MSCs) to trans-differentiate into Schwann-like cells and subsequently tested the MSCs in vivo for their potential to regenerate a transected nerve after implantation. The EBM-MSCs from three equine donors were differentiated into SCLs for 7 days, in vitro, in the presence of specialized differentiation medium and evaluated for morphological characteristics, by using confocal microscopy, and for protein characteristics, by using selected Schwann cell markers (GFAP and S100b). The EBM-MSCs were then implanted into the fascia surrounding the ramus communicans of one fore limb of three healthy horses after a portion of this nerve was excised. The excised portion of the nerve was examined histologically at the time of transection, and stumps of the nerve were examined histologically at day 45 after transplantation. The EBM-MSCs from all donors demonstrated morphological and protein characteristics of those of Schwann cells 7 days after differentiation. Nerves implanted with EBM-MSCs after nerve transection did not show evidence of nerve regeneration at day 45. Examination of peripheral nerves collected 45 days after injury and stem cell treatment revealed no histological differences between nerves treated with MSCs and those treated with isotonic saline solution (controls). The optimal delivery of MSCs and the model suitable to study the efficacy of MSCs in nerve regeneration should be investigated.
Keywords: horse; mesenchymal stromal cells; nerve regeneration; neuropathies; peripheral nerve; stem cells
Rights: © 2016 Cruz Villagrán, Schumacher, Donnell and Dhar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
RMID: 0030056637
DOI: 10.3389/fvets.2016.00080
Appears in Collections:Animal and Veterinary Sciences publications

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