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Type: Journal article
Title: CRLF2-positive B-cell acute lymphoblastic leukemia in adult patients : a single-institution experience
Author: Konoplev, S.
Lu, X.
Konopleva, M.
Jain, N.
Ouyang, J.
Goswami, M.
Roberts, K.
Valentine, M.
Mullighan, C.
Bueso-Ramos, C.
Zweidler-McKay, P.
Jorgensen, J.
Wang, S.
Citation: American Journal of Clinical Pathology, 2017; 147(4):357-363
Publisher: American Society for Clinical Pathology
Issue Date: 2017
ISSN: 0002-9173
Statement of
Sergej Konoplev, Xinyan Lu, Marina Konopleva, Nitin Jain, Juan Ouyang … Charles G. Mullighan … et al.
Abstract: Cytokine receptor-like factor 2 ( CRLF2 ) rearrangement is found in approximately 50% of pediatric Ph-like B-cell acute lymphoblastic leukemia (B-ALL), and around 50% of CRLF2 + cases harbor JAK mutations. We analyzed CRLF2 expression and studied its correlation with CRLF2 rearrangement in adult patients with B-ALL.Multiparameter flow cytometry (MFC) was performed consecutively in 126 patients.CRLF2 overexpression was detected in 30 (27%) patients, 28 (41%) of 69 patients with B-ALL not otherwise specified, 14 (21%) of 67 untreated patients, and 16 (27%) of 59 patients with relapsed B-ALL, with the highest among Hispanic patients (25/55, 45%). Of CRLF2+ cases, 21 (100%) of 21 cases showed CRLF2 rearrangement by fluorescence in situ hybridization, preferentially involving IGH@CRLF2 (15/15). The entire coding region of JAK2 was sequenced in 14 patients with CRLF2+ B-ALL, and nine (64%) were positive for JAK2 mutations.MFC allows a rapid, inexpensive, and reliable detection of B-ALL with CRLF2 rearrangement that would further facilitate testing for JAK2 mutations for targetable therapy.
Keywords: B-lymphoblastic leukemia/lymphoma; BCR-ABL1–like; Ph-like B-ALL; CRLF2; Flow cytometry; FISH; JAK2 mutation
Rights: © American Society for Clinical Pathology, 2017. All rights reserved.
RMID: 0030083432
DOI: 10.1093/AJCP/AQX005
Appears in Collections:Genetics publications

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