Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/113181
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Type: Journal article
Title: Surface chemical gradient affects the differentiation of human adipose-derived stem cells via ERK1/2 signaling pathway
Author: Liu, X.
Shi, S.
Feng, Q.
Bachhuka, A.
He, W.
Huang, Q.
Zhang, R.
Yang, X.
Vasilev, K.
Citation: ACS Applied Materials and Interfaces, 2015; 7(33):18473-18482
Publisher: American Chemical Society
Issue Date: 2015
ISSN: 1944-8244
1944-8252
Statement of
Responsibility: 
Xujie Liu, Shengjun Shi, Qingling Feng, Akash Bachhuka, Wei He, Qianli Huang, Ranran Zhang, Xing Yang, and Krasimir Vasilev
Abstract: To understand the role of surface chemistry on cell behavior and the associated molecular mechanisms, we developed and utilized a surface chemical gradient of amine functional groups by carefully adjusting the gas composition of 1,7-octadiene (OD) and allylamine (AA) of the plasma phase above a moving substrate. The chemical gradient surface used in the present work shows an increasing N/C ratio and wettability from the OD side toward the AA side with no change in surface topography. Under standard culture conditions (with serum), human adipose-derived stem cells (hASCs) adhesion and spreading area increased toward the AA side of the gradient. However, there were no differences in cell behavior in the absence of serum. These results, supported by the trends in proteins adsorption on the gradient surface, demonstrated that surface chemistry affects the response of hASCs through cell-adhesive serum proteins, rather than interacting directly with the cells. The expression of p-ERK and the osteogenic differentiation increased toward the AA side of the gradient, while adipogenic differentiation decreased in the same direction; however, when the activation of ERK1/2 was blocked by PD98059, the levels of osteogenic or adipogenic differentiation on different regions of the chemical gradient were the same. This indicates that ERK1/2 may be an important downstream signaling pathway of surface chemistry directed stem cell fate.
Keywords: Adipose-derived stem cell; plasma polymerization; surface chemical gradient; differentiation; ERK1/2 activation
Description: Published: August 3, 2015
Rights: © 2015 American Chemical Society
RMID: 0030091062
DOI: 10.1021/acsami.5b04635
Grant ID: http://purl.org/au-research/grants/arc/FT100100292
Appears in Collections:Chemistry and Physics publications

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