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https://hdl.handle.net/2440/11318
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dc.contributor.author | Kerry, D. | - |
dc.contributor.author | Dwivedi, P. | - |
dc.contributor.author | Morris, H. | - |
dc.contributor.author | Omdahl, J. | - |
dc.contributor.author | May, B. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Journal of Biological Chemistry, 1996; 271(47):29715-29721 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | http://hdl.handle.net/2440/11318 | - |
dc.description.abstract | Transcription of the CYP24 gene is induced by 1,25-(OH)2D3 through a vitamin D receptor-dependent process. The functional activities of three possible vitamin D response elements (VDREs), located on the antisense strand of the rat CYP24 promoter, were investigated by transient expression of native and mutant promoter constructs in COS-1, JTC-12, and ROS 17/2.8 cells. A putative VDRE with a half-site spacing of 6 base pairs at -249/-232 (VDRE-3) did not contribute to 1,25-(OH)2D3 induced expression in the native promoter, although activity has been reported when the element was fused to the heterologous thymidine kinase promoter. Two VDREs with half-site spacings of 3 base pairs at -150/-136 and -258/-244 (VDRE-1 and VDRE-2, respectively), showed transcriptional synergism in COS-1 cells when treated with 1,25-(OH)2D3 (10(-7) to 10(-11) M). The contribution of both VDREs was hormone-concentration dependent from 10(-10) to 10(-12) M, with VDRE-1 demonstrating greatest sensitivity to 1,25-(OH)2D3. Transactivation by VDRE-1 was always greater than VDRE-2, but the converse was observed for the binding of vitamin D receptor-retinoid X receptor complex by each VDRE in gel mobility shift assays. The synergy observed between VDRE-1 and VDRE-2 may have important implications in cellular responses to different circulating levels of 1,25-(OH)2D3. | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.source.uri | http://dx.doi.org/10.1074/jbc.271.47.29715 | - |
dc.subject | Cell Line | - |
dc.subject | Animals | - |
dc.subject | Rats | - |
dc.subject | Cytochrome P-450 Enzyme System | - |
dc.subject | Steroid Hydroxylases | - |
dc.subject | Vitamin D | - |
dc.subject | Nuclear Proteins | - |
dc.subject | Mutagenesis, Site-Directed | - |
dc.subject | Transcription, Genetic | - |
dc.subject | Gene Expression Regulation, Enzymologic | - |
dc.subject | Protein Binding | - |
dc.subject | Promoter Regions, Genetic | - |
dc.subject | Vitamin D3 24-Hydroxylase | - |
dc.title | Transcriptional Sygernism Between Vitamin D-Rsponsive Elements in the Rat 25-Hyroxyvitamin D-3 24-Hydroxylase (CYP24) Promotor | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1074/jbc.271.47.29715 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Morris, H. [0000-0002-2745-3750] | - |
Appears in Collections: | Aurora harvest 7 Biochemistry publications |
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