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dc.contributor.authorNeufing, P.-
dc.contributor.authorShearwin, K.-
dc.contributor.authorCamerotto, J.-
dc.contributor.authorEgan, J.-
dc.identifier.citationMolecular Microbiology, 1996; 21(4):751-761-
dc.descriptionArticle first published online: 31 OCT 2003-
dc.description.abstractWe have shown previously that the cII gene product of the non-lambdoid temperate bacteriophage 186 is required for the establishment of lysogeny. We show here that CII, a potential helix-turn-helix DNA-binding protein, establishes lysogeny by activating a promoter (p E) which spans the apl/cII intergenic region, upstream of the lysogenic promoter, p L. The start site of the p E transcript (+1) has been mapped by primer extension and we have identified the CII binding determinants at p E by DNase I footprinting. CII binds to inverted repeat sequences separated by two turns of the helix, with binding half-sites centred at the −38 and −58 positions of p E. Oligomerisation studies with purified CII protein indicate that a CII tetramer may be the species that binds to this site. We also show that p E is subject to direct negative feedback by the CI repressor.-
dc.description.statementofresponsibilityPetra J. Neufing, Keith E. Shearwin, Julianne Camerotto and J. Barry Egan-
dc.publisherBlackwell Science-
dc.rights© 1996 Blackwell Science Ltd.-
dc.titleThe CII protein of bacteriophage 186 establishes lysogeny by activating a promoter upstream of the lysogenic promoter-
dc.typeJournal article-
dc.identifier.orcidShearwin, K. [0000-0002-7736-2742]-
Appears in Collections:Aurora harvest 7
Biochemistry publications

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