Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/11399
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Type: Journal article
Title: Analysis of mutations in the creA gene involved in carbon catabolite repression in Aspergillus nidulans
Author: Shroff, R.
Lockington, R.
Kelly, J.
Citation: Canadian Journal of Microbiology, 1996; 42(9):950-959
Publisher: NATL RESEARCH COUNCIL CANADA
Issue Date: 1996
ISSN: 0008-4166
1480-3275
Statement of
Responsibility: 
Robert A. Shroff, Robin A. Lockington and Joan M. Kelly
Abstract: The molecular nature of a number of creA mutant alleles has been determined. Three alleles analysed are missense mutations in the DNA binding domain and predicted to reduce but not abolish binding. Of the other four alleles, two result from frameshifts: one has a nonsense mutilation and the other has an inversion. All four alleles result in truncations of the protein after the zinc finger domain, such that the protein no longer contains at least the carboxy terminal 145 amino acids, so identifying a region required for repression. Transcriptional analysis of creA indicates that the transcript is autoregulated and analysis using 5' rapid amplification of cDNA ends indicates that transcriptional start points exist in clusters over a region of 200 bp located up to 595 bp 5' of the translational start point. The two major clusters have potential CREA-binding sites (SYGGRG) at appropriate positions to allow autoregulation. Autoregulation leads to the creA transcript being most abundant in carbon catabolite nonrepressing conditions, and this, together with the phenotypes of the mutant alleles, has led to the suggestion that CREA has effects under conditions generally not considered as carbon catabolite repressing, as well as in carbon catabolite repressing conditions.
Keywords: carbon catabolite repression; MIG1; CREA; zinc finger protein; transcriptional repressor
RMID: 0030004340
DOI: 10.1139/m96-122
Appears in Collections:Genetics publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.