Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/114054
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorDingle, A.en
dc.contributor.authorYap, K.en
dc.contributor.authorGerrand, Y.en
dc.contributor.authorTaylor, C.en
dc.contributor.authorKeramidaris, E.en
dc.contributor.authorLokmic, Z.en
dc.contributor.authorKong, A.en
dc.contributor.authorPeters, H.en
dc.contributor.authorMorrison, W.en
dc.contributor.authorMitchell, G.en
dc.date.issued2018en
dc.identifier.citationAngiogenesis, 2018; 21(3):581-597en
dc.identifier.issn0969-6970en
dc.identifier.issn1573-7209en
dc.identifier.urihttp://hdl.handle.net/2440/114054-
dc.description.abstractThe liver sinusoidal capillaries play a pivotal role in liver regeneration, suggesting they may be beneficial in liver bioengineering. This study isolated mouse liver sinusoidal endothelial cells (LSECs) and determined their ability to form capillary networks in vitro and in vivo for liver tissue engineering purposes.In vitro LSECs were isolated from adult C57BL/6 mouse livers. Immunofluorescence labelling indicated they were LYVE-1+/CD32b+/FactorVIII+/CD31-. Scanning electron microscopy of LSECs revealed the presence of characteristic sieve plates at 2 days. LSECs formed tubes and sprouts in the tubulogenesis assay, similar to human microvascular endothelial cells (HMEC); and formed capillaries with lumens when implanted in a porous collagen scaffold in vitro. LSECs were able to form spheroids, and in the spheroid gel sandwich assay produced significantly increased numbers (p = 0.0011) of capillary-like sprouts at 24 h compared to HMEC spheroids. Supernatant from LSEC spheroids demonstrated significantly greater levels of vascular endothelial growth factor-A and C (VEGF-A, VEGF-C) and hepatocyte growth factor (HGF) compared to LSEC monolayers (p = 0.0167; p = 0.0017; and p < 0.0001, respectively), at 2 days, which was maintained to 4 days for HGF (p = 0.0017) and VEGF-A (p = 0.0051). In vivo isolated mouse LSECs were prepared as single cell suspensions of 500,000 cells, or as spheroids of 5000 cells (100 spheroids) and implanted in SCID mouse bilateral vascularized tissue engineering chambers for 2 weeks. Immunohistochemistry identified implanted LSECs forming LYVE-1+/CD31- vessels. In LSEC implanted constructs, overall lymphatic vessel growth was increased (not significantly), whilst host-derived CD31+ blood vessel growth increased significantly (p = 0.0127) compared to non-implanted controls. LSEC labelled with the fluorescent tag DiI prior to implantation formed capillaries in vivo and maintained LYVE-1 and CD32b markers to 2 weeks.Isolated mouse LSECs express a panel of vascular-related cell markers and demonstrate substantial vascular capillary-forming ability in vitro and in vivo. Their production of liver growth factors VEGF-A, VEGF-C and HGF enable these cells to exert a growth stimulus post-transplantation on the in vivo host-derived capillary bed, reinforcing their pro-regenerative capabilities for liver tissue engineering studies.en
dc.description.statementofresponsibilityA. M. Dingle, K. K. Yap, Y-W. Gerrand, C. J. Taylor, E. Keramidaris, Z. Lokmic, A. M. Kong, H. L. Peters, W. A. Morrison, G. M. Mitchellen
dc.language.isoenen
dc.publisherSpringeren
dc.rights© Springer Science+Business Media B.V., part of Springer Nature 2018en
dc.subjectCell marker characteristics; Growth factor production; In vitro angiogenesis assays; In vivo implantation into a vascularized tissue engineering chamber; Liver sinusoidal endothelial cells; Spheroid formationen
dc.titleCharacterization of isolated liver sinusoidal endothelial cells for liver bioengineeringen
dc.typeJournal articleen
dc.identifier.rmid0030086975en
dc.identifier.doi10.1007/s10456-018-9610-0en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1023187en
dc.identifier.pubid401647-
pubs.library.collectionPharmacology publicationsen
pubs.library.teamDS10en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
Appears in Collections:Pharmacology publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.