Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/114125
Type: Theses
Title: Temporal gene expression analysis reveals a synergistic effect of combined drought and heat stress in grapevine (Vitis vinifera L.)
Author: Hu, Yikang
Issue Date: 2017
School/Discipline: School of Agriculture, Food and Wine
Abstract: Grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) is widely used for winemaking all over the world. Drought and heat stresses are two of the major abiotic stresses reducing grape quality and yield. However, drought and heat tolerance are still poorly characterized in perennial crops such as grapevine. During this study, stomatal conductance, stem water potential and leaf temperature were measured to determine plant physiological status. RNA-seq technology was used for the analysis of differentially expressed genes (DEGs) of leaf samples between the control and three treatments, which were drought, heat and a combined treatment. Gene expression profiles were grouped by treatments and timepoints. The great majority of unique DEGs were found to be induced by the combined drought and heat treatment. 169 up-regulated genes were induced by drought, 85 by heat and 1218 by the combined treatment; 78 down-regulated genes were induced by drought, 72 by heat and 1427 by the combined treatment. Three potential and significant regulation pathways of stress response were identified based on Gene Ontology (GO) analysis i.e. cytokinin-activated signalling pathway, ion transport pathway and Nitric Oxide-mediated pathway. This study provides preliminary insights into the transcriptomic response to drought and heat stress in grapevine.
Advisor: Rodriguez Lopez, Carlos Marcelino
Dissertation Note: Thesis (M.Bio.(PB)) -- University of Adelaide, Masters of Biotechnology (Plant Biotechnology), School of Agriculture, Food and Wine, 2017.
Keywords: coursework
grapevine
RNA-seq
drought
heat
differentially expressed genes
gene ontology
Provenance: This electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exceptions. If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at: http://www.adelaide.edu.au/legals
Appears in Collections:School of Agriculture, Food and Wine

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