Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/11417
Type: Journal article
Title: Cis-regulatory elements of the mitotic regulator, string/Cdc25
Author: Lehman, D.
Patterson, B.
Johnston, L.
Balzer, T.
Britton, J.
Saint, R.
Edgar, B.
Citation: Development, 1999; 126(9):1793-1803
Publisher: Company of Biologists
Issue Date: 1999
ISSN: 0950-1991
1477-9129
Statement of
Responsibility: 
Dara A. Lehman; Briony Patterson, Laura A. Johnston; Tracy Balzer; Jessica S. Britton; Robert Saint and Bruce A. Edgar
Abstract: Mitosis in most Drosophila cells is triggered by brief bursts of transcription of string (stg), a Cdc25-type phosphatase that activates the mitotic kinase, Cdk1 (Cdc2). To understand how string transcription is regulated, we analyzed the expression of string-lacZ reporter genes covering approximately 40 kb of the string locus. We also tested protein coding fragments of the string locus of 6 kb to 31.6 kb for their ability to complement loss of string function in embryos and imaginal discs. A plethora of cis-acting elements spread over >30 kb control string transcription in different cells and tissue types. Regulatory elements specific to subsets of epidermal cells, mesoderm, trachea and nurse cells were identified, but the majority of the string locus appears to be devoted to controlling cell proliferation during neurogenesis. Consistent with this, compact promotor-proximal sequences are sufficient for string function during imaginal disc growth, but additional distal elements are required for the development of neural structures in the eye, wing, leg and notum. We suggest that, during evolution, cell-type-specific control elements were acquired by a simple growth-regulated promoter as a means of coordinating cell division with developmental processes, particularly neurogenesis.
Keywords: Drosophila; Cis-acting element
Rights: Copyright © The Company of Biologists Ltd 2008
RMID: 0030004327
Published version: http://dev.biologists.org/cgi/content/abstract/126/9/1793
Appears in Collections:Genetics publications

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