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https://hdl.handle.net/2440/11426
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DC Field | Value | Language |
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dc.contributor.author | Todd, R. | - |
dc.contributor.author | Kelly, J. | - |
dc.contributor.author | Davis, M. | - |
dc.contributor.author | Hynes, M. | - |
dc.date.issued | 1997 | - |
dc.identifier.citation | Fungal Genetics and Biology, 1997; 22(2):92-102 | - |
dc.identifier.issn | 1087-1845 | - |
dc.identifier.issn | 1096-0937 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11426 | - |
dc.description.abstract | The facB gene of Aspergillus nidulans encodes a DNA binding transcriptional activator required for growth on acetate as a sole carbon source. FacB contains N-terminal GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA binding and leucine zipper-like heptad repeat motifs and central and C-terminal acidic alpha-helical regions. facB recessive loss of function mutants are deficient in acetate induction of acetyl-CoA synthase, isocitrate lyase, malate synthase, acetamidase, and NADP-isocitrate dehydrogenase. Characterization of lesions in facB mutant alleles has localized important functional regions of the FacB protein. Two extreme mutants are shown to lack the C-terminal region of the protein. Two temperature sensitive mutants contain amino acid substitutions in the DNA binding domain and are shown to affect acetate induction of amdS-lacZ expression and confer temperature sensitive in vitro DNA binding. Two temperature sensitive facB mutations result in thermolability of acetyl-CoA synthase, isocitrate lyase, and malate synthase but not acetamidase or NADP-isocitrate dehydrogenase in crude extracts. This suggests that FacB may have a structural role in acetate metabolism in addition to its regulatory function. | - |
dc.language.iso | en | - |
dc.publisher | Elsevier BV | - |
dc.source.uri | http://dx.doi.org/10.1006/fgbi.1997.1007 | - |
dc.subject | Escherichia coli | - |
dc.subject | Aspergillus nidulans | - |
dc.subject | Acetates | - |
dc.subject | Amidohydrolases | - |
dc.subject | Trans-Activators | - |
dc.subject | Fungal Proteins | - |
dc.subject | Recombinant Fusion Proteins | - |
dc.subject | Sequence Analysis, DNA | - |
dc.subject | Enzyme Stability | - |
dc.subject | Temperature | - |
dc.subject | Enzyme Induction | - |
dc.subject | Sequence Deletion | - |
dc.subject | Amino Acid Sequence | - |
dc.subject | Zinc Fingers | - |
dc.subject | Mutation | - |
dc.subject | Alleles | - |
dc.subject | Genes, Fungal | - |
dc.subject | Molecular Sequence Data | - |
dc.title | Molecular characterisation of mutants of the acetate regulatory gene facB of Aspergillus nidulans | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1006/fgbi.1997.1007 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 2 Genetics publications |
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