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Type: Journal article
Title: Construction of an ~700-kb transcript map around the Familial Mediterranean Fever locus on human chromosome 16p13.3
Author: Centola, M.
Chen, X.
Sood, R.
Deng, Z.
Aksentijevich, I.
Blake, T.
Ricke, D.
Chen, X.
Wood, G.
Zaks, N.
Richards, N.
Krizman, D.
Mansfield, E.
Apostolou, S.
Liu, J.
Shafran, N.
Vedula, A.
Hamon, M.
Cercek, A.
Kahan, T.
et al.
Citation: Genome Research, 1998; 8(11):1172-1191
Issue Date: 1998
ISSN: 1088-9051
Statement of
Michael Centola, Xiaoguang Chen, Raman Sood, Zuoming Deng, Ivona Aksentijevich, Trevor Blake, Darrell O. Ricke, Xiang Chen, Geryl Wood, Nurit Zaks, Neil Richards, David Krizman, Elizabeth Mansfield, Sinoula Apostolou, Jingmei Liu, Neta Shafran, Anil Vedula, Melanie Hamon, Andrea Cercek, Tanaz Kahan, Deborah Gumucio, David F. Callen, Robert I. Richards, Robert K. Moyzis, Norman A. Doggett, Francis S. Collins, P. Paul Liu, Nathan Fischel-Ghodsian and Daniel L. Kastner
Abstract: We used a combination of cDNA selection, exon amplification, and computational prediction from genomic sequence to isolate transcribed sequences from genomic DNA surrounding the familial Mediterranean fever (FMF) locus. Eighty-seven kb of genomic DNA around D16S3370, a marker showing a high degree of linkage disequilibrium with FMF, was sequenced to completion, and the sequence annotated. A transcript map reflecting the minimal number of genes encoded within the ∼700 kb of genomic DNA surrounding the FMF locus was assembled. This map consists of 27 genes with discreet messages detectable on Northerns, in addition to three olfactory-receptor genes, a cluster of 18 tRNA genes, and two putative transcriptional units that have typical intron–exon splice junctions yet do not detect messages on Northerns. Four of the transcripts are identical to genes described previously, seven have been independently identified by the French FMF Consortium, and the others are novel. Six related zinc-finger genes, a cluster of tRNAs, and three olfactory receptors account for the majority of transcribed sequences isolated from a 315-kb FMF central region (betweenD16S468/D16S3070 and cosmid 377A12). Interspersed among them are several genes that may be important in inflammation. This transcript map not only has permitted the identification of the FMF gene (MEFV), but also has provided us an opportunity to probe the structural and functional features of this region of chromosome 16.
Keywords: Chromosomes, Human, Pair 16; Humans; Familial Mediterranean Fever; Receptors, Odorant; DNA; DNA, Complementary; RNA, Transfer; Physical Chromosome Mapping; Cloning, Molecular; Sequence Analysis, DNA; Transcription, Genetic; Gene Amplification; Amino Acid Sequence; Zinc Fingers; Sequence Homology, Amino Acid; Genes; Multigene Family; Genome, Human; Exons; Molecular Sequence Data
Rights: Copyright © 1998 by Cold Spring Harbor Laboratory Press
RMID: 0030004313
DOI: 10.1101/gr.8.11.1172
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Appears in Collections:Genetics publications

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