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Type: Journal article
Title: A molecular and evolutionary study of the β-globin gene family of the Australian marsupial Sminthopsis Crassicaudata
Other Titles: A molecular and evolutionary study of the beta-globin gene family of the Australian marsupial Sminthopsis crassicaudata
Author: Cooper, S.
Murphy, R.
Dolman, G.
Hussey, D.
Hope, R.
Citation: Molecular Biology and Evolution, 1996; 13(7):1012-1022
Issue Date: 1996
ISSN: 0737-4038
Statement of
S J Cooper, R Murphy, G Dolman, D Hussey and R M Hope
Abstract: Beta-globin gene families in eutherians (placental mammals) consist of a set of four or more developmentally regulated genes which are closely linked and, in general, arranged in the order 5'-embryonic/fetal genes-adult genes-3'. This cluster of genes is proposed to have arisen by tandem duplication of ancestral beta-globin genes, with the first duplication occurring 200 to 155 MYBP just prior to a period in mammalian evolution when eutherians and marsupials diverged from a common ancestor. In this paper we trace the evolutionary history of the beta-globin gene family back to the origins of these mammals by molecular characterization of the beta-globin gene family of the Australian marsupial Sminthopsis crassicaudata. Using Southern and restriction analysis of total genomic DNA and bacteriophage clones of beta-like globin genes, we provide evidence that just two functional beta-like globin genes exist in this marsupial, including one embryonic-expressed gene (S.c-epsilon) and one adult-expressed gene (S.c-beta), linked in the order 5'-epsilon-beta-3'. The entire DNA sequence of the adult beta-globin gene is reported and shown to be orthologous to the adult beta-globin genes of the North American marsupial Didelphis virginiana and eutherian mammals. These results, together with results from a phylogenetic analysis of mammalian beta-like globin genes, confirm the hypothesis that a two-gene cluster, containing an embryonic- and an adult-expressed beta-like globin gene, existed in the most recent common ancester of marsupials and eutherians. Northern analysis of total RNA isolated from embryos and neonatals indicates that a switch from embryonic to adult gene expression occurs at the time of birth, coinciding with the transfer of the marsupial from a uterus to a pouch environment.
Keywords: Animals; Marsupialia; Deoxyribonuclease BamHI; Site-Specific DNA-Methyltransferase (Adenine-Specific); Globins; DNA Probes; Blotting, Northern; Blotting, Southern; In Situ Hybridization; Restriction Mapping; Cloning, Molecular; Age Factors; Evolution, Molecular; Phylogeny; Gene Expression Regulation, Developmental; Amino Acid Sequence; Base Sequence; Multigene Family; Molecular Sequence Data; Australia; Genetic Variation; Genetic Linkage
Rights: © 1996 Society for Molecular Biology and Evolution
RMID: 0030004281
DOI: 10.1093/oxfordjournals.molbev.a025651
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