Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||A molecular and evolutionary study of the β-globin gene family of the Australian marsupial Sminthopsis Crassicaudata|
|Other Titles:||A molecular and evolutionary study of the beta-globin gene family of the Australian marsupial Sminthopsis crassicaudata|
|Citation:||Molecular Biology and Evolution, 1996; 13(7):1012-1022|
|Publisher:||SOC MOLECULAR BIOLOGY EVOLUTION|
|S J Cooper, R Murphy, G Dolman, D Hussey and R M Hope|
|Abstract:||Beta-globin gene families in eutherians (placental mammals) consist of a set of four or more developmentally regulated genes which are closely linked and, in general, arranged in the order 5'-embryonic/fetal genes-adult genes-3'. This cluster of genes is proposed to have arisen by tandem duplication of ancestral beta-globin genes, with the first duplication occurring 200 to 155 MYBP just prior to a period in mammalian evolution when eutherians and marsupials diverged from a common ancestor. In this paper we trace the evolutionary history of the beta-globin gene family back to the origins of these mammals by molecular characterization of the beta-globin gene family of the Australian marsupial Sminthopsis crassicaudata. Using Southern and restriction analysis of total genomic DNA and bacteriophage clones of beta-like globin genes, we provide evidence that just two functional beta-like globin genes exist in this marsupial, including one embryonic-expressed gene (S.c-epsilon) and one adult-expressed gene (S.c-beta), linked in the order 5'-epsilon-beta-3'. The entire DNA sequence of the adult beta-globin gene is reported and shown to be orthologous to the adult beta-globin genes of the North American marsupial Didelphis virginiana and eutherian mammals. These results, together with results from a phylogenetic analysis of mammalian beta-like globin genes, confirm the hypothesis that a two-gene cluster, containing an embryonic- and an adult-expressed beta-like globin gene, existed in the most recent common ancester of marsupials and eutherians. Northern analysis of total RNA isolated from embryos and neonatals indicates that a switch from embryonic to adult gene expression occurs at the time of birth, coinciding with the transfer of the marsupial from a uterus to a pouch environment.|
|Keywords:||Animals; Marsupialia; Deoxyribonuclease BamHI; Site-Specific DNA-Methyltransferase (Adenine-Specific); Globins; DNA Probes; Blotting, Northern; Blotting, Southern; In Situ Hybridization; Restriction Mapping; Cloning, Molecular; Age Factors; Evolution, Molecular; Phylogeny; Gene Expression Regulation, Developmental; Amino Acid Sequence; Base Sequence; Multigene Family; Molecular Sequence Data; Australia; Genetic Variation; Genetic Linkage|
|Rights:||© 1996 Society for Molecular Biology and Evolution|
|Appears in Collections:||Genetics publications|
Environment Institute Leaders publications
Environment Institute publications
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.