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https://hdl.handle.net/2440/11481
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dc.contributor.author | Cooper, S. | - |
dc.contributor.author | Murphy, R. | - |
dc.contributor.author | Dolman, G. | - |
dc.contributor.author | Hussey, D. | - |
dc.contributor.author | Hope, R. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Molecular Biology and Evolution, 1996; 13(7):1012-1022 | - |
dc.identifier.issn | 0737-4038 | - |
dc.identifier.issn | 1537-1719 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11481 | - |
dc.description.abstract | Beta-globin gene families in eutherians (placental mammals) consist of a set of four or more developmentally regulated genes which are closely linked and, in general, arranged in the order 5'-embryonic/fetal genes-adult genes-3'. This cluster of genes is proposed to have arisen by tandem duplication of ancestral beta-globin genes, with the first duplication occurring 200 to 155 MYBP just prior to a period in mammalian evolution when eutherians and marsupials diverged from a common ancestor. In this paper we trace the evolutionary history of the beta-globin gene family back to the origins of these mammals by molecular characterization of the beta-globin gene family of the Australian marsupial Sminthopsis crassicaudata. Using Southern and restriction analysis of total genomic DNA and bacteriophage clones of beta-like globin genes, we provide evidence that just two functional beta-like globin genes exist in this marsupial, including one embryonic-expressed gene (S.c-epsilon) and one adult-expressed gene (S.c-beta), linked in the order 5'-epsilon-beta-3'. The entire DNA sequence of the adult beta-globin gene is reported and shown to be orthologous to the adult beta-globin genes of the North American marsupial Didelphis virginiana and eutherian mammals. These results, together with results from a phylogenetic analysis of mammalian beta-like globin genes, confirm the hypothesis that a two-gene cluster, containing an embryonic- and an adult-expressed beta-like globin gene, existed in the most recent common ancester of marsupials and eutherians. Northern analysis of total RNA isolated from embryos and neonatals indicates that a switch from embryonic to adult gene expression occurs at the time of birth, coinciding with the transfer of the marsupial from a uterus to a pouch environment. | - |
dc.description.statementofresponsibility | S J Cooper, R Murphy, G Dolman, D Hussey and R M Hope | - |
dc.language.iso | en | - |
dc.publisher | SOC MOLECULAR BIOLOGY EVOLUTION | - |
dc.rights | © 1996 Society for Molecular Biology and Evolution | - |
dc.source.uri | http://mbe.oxfordjournals.org.proxy.library.adelaide.edu.au/content/13/7/1012.abstract | - |
dc.subject | Animals | - |
dc.subject | Marsupialia | - |
dc.subject | Deoxyribonuclease BamHI | - |
dc.subject | Site-Specific DNA-Methyltransferase (Adenine-Specific) | - |
dc.subject | Globins | - |
dc.subject | DNA Probes | - |
dc.subject | Blotting, Northern | - |
dc.subject | Blotting, Southern | - |
dc.subject | In Situ Hybridization | - |
dc.subject | Restriction Mapping | - |
dc.subject | Cloning, Molecular | - |
dc.subject | Age Factors | - |
dc.subject | Evolution, Molecular | - |
dc.subject | Phylogeny | - |
dc.subject | Gene Expression Regulation, Developmental | - |
dc.subject | Amino Acid Sequence | - |
dc.subject | Base Sequence | - |
dc.subject | Multigene Family | - |
dc.subject | Molecular Sequence Data | - |
dc.subject | Australia | - |
dc.subject | Genetic Variation | - |
dc.subject | Genetic Linkage | - |
dc.title | A molecular and evolutionary study of the β-globin gene family of the Australian marsupial Sminthopsis Crassicaudata | - |
dc.title.alternative | A molecular and evolutionary study of the beta-globin gene family of the Australian marsupial Sminthopsis crassicaudata | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1093/oxfordjournals.molbev.a025651 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Cooper, S. [0000-0002-7843-8438] | - |
dc.identifier.orcid | Dolman, G. [0000-0001-7611-6841] | - |
Appears in Collections: | Aurora harvest 2 Environment Institute Leaders publications Environment Institute publications Genetics publications |
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