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|Title:||Experience with newer techniques for the laboratory detection of Mycoplasma pneumoniae infection: Adelaide, 1978–1992|
|Citation:||Clinical Infectious Diseases, 1993; 17(Suppl. 1):S90-S99|
|Publisher:||University of Chicago Press|
|B. P. Marmion, J. Williamson, D. A. Worswick, T.-W. Kok, and R. J. Harris|
|Abstract:||Efforts to improve laboratory diagnostic methods for infection due to Mycoplasma pneumoniae have involved the use of a cell-sheet culture method and a modified indirect hemagglutination method for IgM antibody, while direct detection of mycoplasma has employed antigen capture-enzyme immunoassay (Ag-EIA) and polymerase chain reaction (PCR) amplification of sequences within the P1 and 16S ribosomal RNA genes and quantification of the amplified DNA by dot blot hybridization (DBH). Cell-sheet culture was slightly more sensitive and more rapid than culture with cell-free diphasic medium. Indirect hemagglutination detection of IgM antibody to M. pneumoniae was more sensitive than CF and EIA for detection of IgM antibody to mycoplasma. Ag-EIA gave a rapid and reasonably sensitive indication of infection and correlated well with a serological response of patients indicating a current infection. PCR-DBH was a highly sensitive substitute for culture of mycoplasma. Both Ag-EIA and PCR-DBH require confirmation by assessment of serological response to verify that the infection is current and that positive results of PCR-DBH, in particular, are not the result of continuing carriage of the organism from a previous infection, unrelated to the current episode under investigation.|
|Keywords:||Mycoplasma pneumoniae; Pneumonia, Mycoplasma; Antigens, Bacterial|
|Rights:||© 1993 by The University of Chicago. All rights reserved.|
|Appears in Collections:||Medicine publications|
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