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https://hdl.handle.net/2440/115955
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Type: | Journal article |
Title: | International collaborative ring trial of four gene-specific loop-mediated isothermal amplification assays in GMO analysis |
Author: | Li, R. Shi, J. Liu, B. Zhang, D. Zhao, X. Yang, L. |
Citation: | Food Control, 2018; 84:278-283 |
Publisher: | Elsevier |
Issue Date: | 2018 |
ISSN: | 0956-7135 1873-7129 |
Statement of Responsibility: | Rong Li, Jianxin Shi, Biao Liu, Dabing Zhang, Xiangxiang Zhao, Litao Yang |
Abstract: | We performed an international collaborative ring trial to validate gene-specific loop-mediated isothermal amplification (LAMP) assays targeting four exogenous transgenes commonly utilized in the development of genetically modified (GM) crops: bar, pat, cp4-epsps, and Cry1Ac, respectively. A total of 12 laboratories participated in the ring trial and returned their test results. Statistical analysis of the returned results showed high specificity and low limit of detection (LOD) of the four LAMP assays. The LOD for cp4-epsps, Cry1Ac, bar and pat genes assay was 5, 10, 20 and 20 haploid genome equivalents (HGE), respectively. Furthermore, good performance of the four assays was further demonstrated in the analysis of practical samples in which all participating laboratories returned expected and accurate test results. Altogether, the ring trial results confirmed that the four gene-specific LAMP assays can be utilized for quick screening of transgenic traits in routine laboratory analysis. |
Keywords: | Collaborative ring trial; loop-mediated isothermal amplification; gene-specific |
Rights: | © 2017 Elsevier Ltd. All rights reserved. |
DOI: | 10.1016/j.foodcont.2017.08.012 |
Grant ID: | 2016ZX08012-003 2016ZX08012-005 2016ZX08012-002 |
Published version: | http://dx.doi.org/10.1016/j.foodcont.2017.08.012 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 8 |
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