Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/116057
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Type: Journal article
Title: ifet-1 is a broad-scale translational repressor required for normal P granule formation in C. elegans
Author: Sengupta, M.
Low, W.
Patterson, J.
Kim, H.-.M.
Traven, A.
Beilharz, T.
Colaiacovo, M.
Schisa, J.
Boag, P.
Citation: Journal of Cell Science, 2013; 126(3):850-859
Publisher: The Company of Biologists
Issue Date: 2013
ISSN: 0021-9533
1477-9137
Statement of
Responsibility: 
Madhu S. Sengupta, Wai Yee Low, Joseph R. Patterson, Hyun-Min Kim, Ana Traven, Traude H. Beilharz, Monica P. Colaia, covo, Jennifer A. Schisa, and Peter R. Boag
Abstract: Large cytoplasmic ribonucleoprotein germ granule complexes are a common feature in germ cells. In C. elegans these are called P granules and for much of the life-cycle they associate with nuclear pore complexes in germ cells. P granules are rich in proteins that function in diverse RNA pathways. Here we report that the C. elegans homolog of the eIF4E-transporter IFET-1 is required for oogenesis but not spermatogenesis. We show that IFET-1 is required for translational repression of several maternal mRNAs in the distal gonad and functions in conjunction with the broad-scale translational regulators CGH-1, CAR-1 and PATR-1 to regulate germ cell sex determination. Furthermore we have found that IFET-1 localizes to P granules throughout the gonad and in the germ cell lineage in the embryo. Interestingly, IFET-1 is required for the normal ultrastructure of P granules and for the localization of CGH-1 and CAR-1 to P granules. Our findings suggest that IFET-1 is a key translational regulator and is required for normal P granule formation.
Keywords: eIF4E transporter; P granule; germ granules; translational regulation; caenorhabditis elegans
Rights: © 2013. Published by The Company of Biologists Ltd
RMID: 0030075947
DOI: 10.1242/jcs.119834
Grant ID: http://purl.org/au-research/grants/nhmrc/606575
Appears in Collections:Animal and Veterinary Sciences publications

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