Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/116815
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Type: Journal article
Title: Depletion of ribosomal protein S19 causes a reduction of rRNA synthesis
Author: Juli, G.
Gismondi, A.
Monteleone, V.
Caldarola, S.
Iadevaia, V.
Aspesi, A.
Dianzani, I.
Proud, C.
Loreni, F.
Citation: Scientific Reports, 2016; 6(1):35026-1-35026-10
Publisher: Nature Publishing Group
Issue Date: 2016
ISSN: 2045-2322
2045-2322
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Responsibility: 
Giada Juli, Angelo Gismondi, Valentina Monteleone, Sara Caldarola, Valentina Iadevaia, Anna Aspesi, Irma Dianzani, Christopher G. Proud, Fabrizio Loreni
Abstract: Ribosome biogenesis plays key roles in cell growth by providing increased capacity for protein synthesis. It requires coordinated production of ribosomal proteins (RP) and ribosomal RNA (rRNA), including the processing of the latter. Here, we show that, the depletion of RPS19 causes a reduction of rRNA synthesis in cell lines of both erythroid and non-erythroid origin. A similar effect is observed upon depletion of RPS6 or RPL11. The deficiency of RPS19 does not alter the stability of rRNA, but instead leads to an inhibition of RNA Polymerase I (Pol I) activity. In fact, results of nuclear run-on assays and ChIP experiments show that association of Pol I with the rRNA gene is reduced in RPS19-depleted cells. The phosphorylation of three known regulators of Pol I, CDK2, AKT and AMPK, is altered during ribosomal stress and could be involved in the observed downregulation. Finally, RNA from patients with Diamond Blackfan Anemia (DBA), shows, on average, a lower level of 47S precursor. This indicates that inhibition of rRNA synthesis could be one of the molecular alterations at the basis of DBA.
Keywords: Ribosomal proteins
Rights: © The Author(s) 2016. This work is Iicensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
RMID: 0030056735
DOI: 10.1038/srep35026
Appears in Collections:Biochemistry publications

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