Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/117320
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorŁuksza, M.en
dc.contributor.authorKanaan, J.en
dc.contributor.authorMarthiens, V.en
dc.contributor.authorLane, S.en
dc.contributor.authorJones, K.en
dc.contributor.authorTerret, M.en
dc.contributor.authorBasto, R.en
dc.contributor.authorVerlhac, M.en
dc.date.issued2018en
dc.identifier.citationThe Journal of Cell Biology, 2018; 217(10):3416-3430en
dc.identifier.issn0021-9525en
dc.identifier.issn1540-8140en
dc.identifier.urihttp://hdl.handle.net/2440/117320-
dc.description.abstractMouse female meiotic spindles assemble from acentriolar microtubule-organizing centers (aMTOCs) that fragment into discrete foci. These are further sorted and clustered to form spindle poles, thus providing balanced forces for faithful chromosome segregation. To assess the impact of aMTOC biogenesis on spindle assembly, we genetically induced their precocious fragmentation in mouse oocytes using conditional overexpression of Plk4, a master microtubule-organizing center regulator. Excessive microtubule nucleation from these fragmented aMTOCs accelerated spindle assembly dynamics. Prematurely formed spindles promoted the breakage of three different fragilized bivalents, generated by the presence of recombined Lox P sites. Reducing the density of microtubules significantly diminished the extent of chromosome breakage. Thus, improper spindle forces can lead to widely described yet unexplained chromosomal structural anomalies with disruptive consequences on the ability of the gamete to transmit an uncorrupted genome.en
dc.description.statementofresponsibilityMarion Manil-Ségalen, Małgorzata Łuksza, Joanne Kanaan, Véronique Marthiens, Simon I.R. Lane, Keith T. Jones, Marie-Emilie Terret, Renata Basto, Marie-Hélène Verlhacen
dc.language.isoenen
dc.publisherRockefeller University Pressen
dc.rights© 2018 Manil-Ségalen et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https:// creativecommons .org/ licenses/ by/ 4 .0/ ).en
dc.subjectOocytes; Chromosomes, Mammalian; Microtubule-Organizing Center; Animals; Mice, Transgenic; Mice; Meiosis; Female; Spindle Apparatus; Gene Editingen
dc.titleChromosome structural anomalies due to aberrant spindle forces exerted at gene editing sites in meiosisen
dc.typeJournal articleen
dc.identifier.rmid0030099117en
dc.identifier.doi10.1083/jcb.201806072en
dc.identifier.pubid440617-
pubs.library.collectionGenetics publicationsen
pubs.library.teamDS10en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidJones, K. [0000-0002-0294-0851]en
Appears in Collections:Genetics publications

Files in This Item:
File Description SizeFormat 
hdl_117320.pdfPublished version2.77 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.