Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/117386
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Type: Journal article
Title: Detection of N-glycolylneuraminic acid biomarkers in sera from patients with ovarian cancer using an engineered N-glycolylneuraminic acid-specific lectin SubB2M
Author: Shewell, L.
Wang, J.
Paton, J.
Paton, A.
Day, C.
Jennings, M.
Citation: Biochemical and Biophysical Research Communications, 2018; 507(1-4):173-177
Publisher: Elsevier
Issue Date: 2018
ISSN: 0006-291X
1090-2104
Statement of
Responsibility: 
L.K. Shewell, J.J. Wang, J.C. Paton, A.W. Paton, C.J. Day, M.P. Jennings
Abstract: N-glycolylneuraminic acid (Neu5Gc)-containing glycans are a prominent form of aberrant glycosylation found in human tumor cells and have been proposed as cancer biomarkers. The B subunit of the subtilase cytotoxin (SubB) produced by Shiga toxigenic Escherichia coli recognises Neu5Gc containing glycans. We have previously engineered this lectin, SubB2M, for greater specificity and enhanced recognition of Neu5Gc-containing glycans. Here we further explore the utility of SubB2M to detect Neu5Gc tumor biomarkers in sera from patients with ovarian cancer. Using surface plasmon resonance (SPR) we show that SubB2M can detect the established ovarian cancer biomarker, CA125, in a highly sensitive and specific fashion in the context of human serum. These studies established conditions for screening serum samples from patients with ovarian cancer for Neu5Gc glycans. We found that serum from patients with all stages of ovarian cancer had significantly elevated mean levels of Neu5Gc glycans compared to normal controls. Serum from patients with late stage disease (stages IIIC, IV) had uniformly elevated levels of Neu5Gc glycans. Detection of Neu5Gc-glycans using SubB2M has the potential to be used as a diagnostic ovarian cancer biomarker, as well as a tool for monitoring treatment and disease progression in late stage disease.
Keywords: Ovarian cancer; biomarker; N-glycolylneuraminic acid; Neu5Gc; lectin; diagnostic
Rights: © 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
RMID: 0030102974
DOI: 10.1016/j.bbrc.2018.11.001
Grant ID: http://purl.org/au-research/grants/nhmrc/1071659
http://purl.org/au-research/grants/nhmrc/1084050
http://purl.org/au-research/grants/nhmrc/1138466
Appears in Collections:Molecular and Biomedical Science publications

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