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dc.contributor.authorKittipassorn, T.en
dc.contributor.authorHaydinger, C.en
dc.contributor.authorWood, J.en
dc.contributor.authorMammone, T.en
dc.contributor.authorCasson, R.en
dc.contributor.authorPeet, D.en
dc.identifier.citationExperimental Eye Research, 2019; 181:127-135en
dc.description.abstractMüller cells (MCs) play a crucial role in the retina, and cultured MC lines are an important tool with which to study MC function. Transformed MC lines have been widely used; however, the transformation process can also lead to unwanted changes compared to the primary cells from which they were derived. To provide an alternative experimental tool, a monoclonal novel spontaneously immortalized rat Müller cell line, SIRMu-1, was derived from primary rat MCs and characterized. Immunofluorescence, western blotting and RNA sequencing demonstrate that the SIRMu-1 cell line retains similar characteristics to cultured primary MCs in terms of expression of the MC markers cellular retinaldehyde-binding protein, glutamine synthetase, S100, vimentin and glial fibrillary acidic protein at both the mRNA and protein levels. Both the cellular morphology and overall transcriptome of the SIRMu-1 cells are more similar to primary rat MCs than the commonly used rMC-1 cells, a well-described, transformed rat MC line. Furthermore, SIRMu-1 cells proliferate rapidly, have an effectively indefinite life span and a high transfection efficiency. The expression of Y chromosome specific genes confirmed that the SIRMu-1 cells are derived from male MCs. Thus, the SIRMu-1 cell line represents a valuable experimental tool to study roles of MCs in both physiological and pathological states.en
dc.description.statementofresponsibilityThaksaon Kittipassorn, Cameron D. Haydinger, John P.M. Wood, Teresa Mammone, Robert J. Casson, Daniel J. Peeten
dc.rights© 2019 Elsevier Ltd. All rights reserved.en
dc.subjectCell culture; Müller cell; Retina; SIRMu-1; Spontaneously immortalized cellen
dc.titleCharacterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1en
dc.typeJournal articleen
pubs.library.collectionBiochemistry publicationsen
dc.identifier.orcidKittipassorn, T. [0000-0001-9854-2905]en
dc.identifier.orcidCasson, R. [0000-0003-2822-4076]en
dc.identifier.orcidPeet, D. [0000-0002-6085-8936]en
Appears in Collections:Biochemistry publications

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