Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/11765
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Increased bone resorption precedes bone formation in the ovariectomised rat |
Author: | Sims, N. Morris, H. Moore, R. Durbridge, T. |
Citation: | Calcified Tissue International, 1996; 59(2):112-127 |
Publisher: | SPRINGER |
Issue Date: | 1996 |
ISSN: | 0171-967X 1432-0827 |
Statement of Responsibility: | N. A. Sims, H. A. Morris, R. J. Moore, T. C. Durbridge |
Abstract: | This study describes an increase in biochemical and histomorphometric markers of bone resorption prior to increased bone formation and trabecular bone loss in the ovariectomized rat. Six-month-old, female Sprague Dawley rats were either sham operated or ovariectomized (Ovx) and killed at 0, 6, 9, 15, 18, 21, and 42 days postoperation when femora were collected and trabecular bone volume (BV/TV) was determined from von Kossa silver-stained sections using the Quantimet 520 image analysis system in the distal region. A number of these sections were also examined unstained for fluorochrome labels, and stained for acid phosphatase to detect osteoclast-like cells (ACP surface). At 18 days postoperation, lumbar vertebrae were examined. Blood and urine specimens were analyzed for bone-related biochemical variables. ACP surface was significantly greater in Ovx rats compared with sham at 6 days postoperation (mean ACP surface (%TS) +/- SEM: sham 36.4 +/- 1.9; Ovx 40.3 +/- 1.2, P < 0.05) as was urinary hydroxyproline excretion. Serum osteocalcin and alkaline phosphatase activity were not elevated in Ovx rats compared with Sham until 9 days postoperation. Mineral apposition rate (MAR) was increased at 12 days after ovariectomy (mean MAR (microm/day) +/- SEM: sham 0.85 +/- 0.06; Ovx 1.23 +/- 0.06, P < 0.05). Trabecular bone volume (BV/TV) at a specific site in the metaphyseal-diaphyseal core area was significantly lower at 15 days postoperation (mean (%) +/- SEM: Sham 7.40 +/- 1.23, Ovx 4.25 0 0.65, P < 0.05). There was no difference in lumbar vertebral BV/TV between the two groups at 18 days postoperation, however, ACP surface was elevated in the Ovx rats (P < 0.05). A systemic increase in bone resorption at 6 days postovariectomy precedes increased formation whereas the length of time required for the dissolution of trabeculae postoperation is determined locally. |
Keywords: | Femur Lumbar Vertebrae Osteoclasts Animals Humans Rats Rats, Sprague-Dawley Osteoporosis, Postmenopausal Bone Resorption Disease Models, Animal Estradiol Fluorescent Dyes Radioimmunoassay Ovariectomy Bone Remodeling Bone Development Bone Density Female Biomarkers |
DOI: | 10.1007/s002239900098 |
Published version: | http://dx.doi.org/10.1007/s002239900098 |
Appears in Collections: | Aurora harvest 7 Physiology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.