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Type: Journal article
Title: NIPAM-based microgel microenvironment regulates the therapeutic function of cardiac stromal cells
Author: Cui, X.
Tang, J.
Hartanto, Y.
Zhang, J.
Bi, J.
Dai, S.
Qiao, S.
Cheng, K.
Zhang, H.
Citation: ACS Applied Materials and Interfaces, 2018; 10(44):37783-37796
Publisher: American Chemical Society
Issue Date: 2018
ISSN: 1944-8244
Statement of
Xiaolin Cui, Junnan Tang, Yusak Hartanto, Jiabin Zhang, Jingxiu Bi, Sheng Dai, Shi Zhang Qiao, Ke Cheng, and Hu Zhang
Abstract: To tune the chemical, physical, and mechanical microenvironment for cardiac stromal cells to treat acute myocardial infarction (MI), we prepared a series of thermally responsive microgels with different surface charges (positive, negative, and neutral) and different degrees of hydrophilicity, as well as functional groups (carboxyl, hydroxyl, amino, and methyl). These microgels were used as injectable hydrogels to create an optimized microenvironment for cardiac stromal cells (CSCs). Our results indicated that a hydrophilic and negatively charged microenvironment created from poly( N-isopropylacrylamide- co-itaconic acid) was favorable for maintaining high viability of CSCs, promoting CSC proliferation and facilitating the formation of CSC spheroids. A large number of growth factors, such as vascular endothelial growth factor (VEGF), insulin-like growth factor I (IGF-1), and stromal-derived factor-1 (SDF-1) were released from the spheroids, promoting neonatal rat cardiomyocyte activation and survival. After injecting the poly( N-isopropylacrylamide- co-itaconic acid) microgel into mice, we examined their acute inflammation and T-cell immune reactions. The microgel itself did not elicit obvious immune response. We then injected the same microgel-encapsulated with CSCs into MI mice. The result revealed the treatment-promoted MI heart repair through angiogenesis and inhibition of apoptosis with an improved cell retention rate. This study will open a door for tailoring poly( N-isopropylacrylamide)-based microgel as a delivery vehicle for CSC therapy.
Keywords: Injectable hydrogels; NIPAM microgel; thermo-responsive microgel; cardiac stromal cells; multicellular spheroids; heart repair
Rights: © 2018 American Chemical Society
RMID: 0030101947
DOI: 10.1021/acsami.8b09757
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Appears in Collections:Chemical Engineering publications

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