Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/11804
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dc.contributor.authorMcMillen, I.en
dc.contributor.authorWarnes, K.en
dc.contributor.authorAdams, M.en
dc.contributor.authorRobinson, J.en
dc.contributor.authorOwens, J.en
dc.contributor.authorCoulter, C.en
dc.date.issued2000en
dc.identifier.citationEndocrinology, 2000; 141(2):539-543en
dc.identifier.issn0013-7227en
dc.identifier.issn1945-7170en
dc.identifier.urihttp://hdl.handle.net/2440/11804-
dc.description.abstractWe have investigated the effects of fetal growth restriction, induced by restriction of placental growth and function (PR), on 11beta-hydroxysteroid dehydrogenase type 1 (11betaHSD-1) and 11betaHSD-2 messenger RNA (mRNA) expression in fetal tissues in the sheep, using Northern blot analysis. Fetal liver, kidney, and adrenals were collected from normally grown fetuses at 90 days (n = 6), 125 days (n = 6), and 141-145 days (n = 7) and from PR fetuses at 141-145 days (n = 6). Expression of 11betaHSD-1 mRNA in the fetal liver increased significantly between 125 days (7.4+/-0.8) and 141-145 days gestation (27+/-5.3). There was also an approximately 2-fold increase in the ratio of 11betaHSD-1 mRNA/18S rRNA expression in the PR group (53.8+/-7.9) compared with that in control animals at 141-145 days gestation. There was a significant decrease in 11betaHSD-2 mRNA in fetal adrenals between 125 days (41.6+/-2.4) and 141-145 days (26.7+/-1.1) gestation, but there was no effect of PR on the expression of adrenal 11betaHSD-2 mRNA. 11betaHSD-2 mRNA expression in the fetal kidney increased between 90 days (16.8+/-1.7) and 141-145 days gestation (31.7+/-4.3), but there was no effect of PR on the levels of 11betaHSD-2 mRNA in the fetal kidney. In summary, 11betaHSD-2 mRNA is differentially regulated in the fetal adrenal and kidney in the sheep fetus during late gestation. There is also a specific increase in the expression of 11betaHSD-1 mRNA in the liver of growth-restricted fetuses in late gestation. This suggests that there is increased hepatic exposure to cortisol in the growth-restricted fetus, which may be important in the reprogramming of hepatic physiology that occurs after growth restriction in utero.en
dc.language.isoenen
dc.publisherEndocrine Socen
dc.subjectLiver; Kidney; Adrenal Glands; Placenta; Animals; Sheep; Fetal Growth Retardation; Body Weight; Isoenzymes; Hydroxysteroid Dehydrogenases; 11-beta-Hydroxysteroid Dehydrogenase Type 1; RNA, Messenger; Transcription, Genetic; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Embryonic and Fetal Development; Gestational Age; Pregnancy; Femaleen
dc.titleImpact of restriction of placental and fetal growth on expression of 11b-hydroxysteroid dehydrogenase type 1 and type 2 messenger ribonucleic acid in the liver, kidney, and adrenal of the sheep fetusen
dc.typeJournal articleen
dc.identifier.rmid0001001672en
dc.identifier.doi10.1210/endo.141.2.7338en
dc.identifier.pubid63082-
pubs.library.collectionPhysiology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidRobinson, J. [0000-0002-4515-6039]en
dc.identifier.orcidOwens, J. [0000-0002-7498-1353]en
Appears in Collections:Physiology publications

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