Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/11972
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dc.contributor.authorHardingham, J.en
dc.contributor.authorKotasek, D.en
dc.contributor.authorSage, R.en
dc.contributor.authorEaton, M.en
dc.contributor.authorPascoe, V.en
dc.contributor.authorDobrovic, A.en
dc.date.issued1995en
dc.identifier.citationMolecular Medicine, 1995; 1(7):789-794en
dc.identifier.issn1076-1551en
dc.identifier.issn1528-3658en
dc.identifier.urihttp://hdl.handle.net/2440/11972-
dc.description.abstractRecurrent and metastatic carcinoma of the colorectum remains a major problem, with survival at 5 years post curative resection still only about 50%. Moreover, up to 30% of patients who present with early stage disease also relapse and die within 5 years, suggesting the presence of micrometastatic disease at diagnosis. One route of metastatic spread is via the blood stream, hence the detection of tumor cells in blood is likely to provide an important predictive tool with respect to relapse of disease. We have developed a sensitive molecular technique to identify tumor cells in blood using mutations in codon 12 of the K-ras gene as a marker.Twenty-seven patients whose tumor carried a mutation in codon 12 of K-ras were studied for the presence of tumor cells in perioperative peripheral blood samples. Immunomagnetic beads, labeled with an epithelial-specific antibody, were used to harvest epithelial cells from blood. K-ras mutations were identified in this selected population using a polymerase chain reaction (PCR)-based analysis (immunobead-PCR).Circulating K-ras mutant cells were detected in 9 or 27 patients; seven of these nine patients have since died due to recurrent or metastatic disease. Mutant cells were not detected in 18 patients, and 16 or 18 have remained disease free (median follow-up: 16 months; range: 7-42 months). Kaplan-Meier analysis showed that detection of K-ras mutant cells in bloods was associated with significantly reduced disease-free survival (p = 0.0001).This study indicates that detection of circulating tumor cells perioperatively by immunobead-PCR provides a sensitive prognostic marker for recurrent and metastatic colorectal cancer.en
dc.language.isoenen
dc.publisherBLACKWELL SCIENCE PUBL INC CAMBRIDGEen
dc.subjectHumans; Carcinoma; Colorectal Neoplasms; Neoplasm Metastasis; Recurrence; Disease-Free Survival; Polymerase Chain Reaction; Mutation; Genes, ras; Aged; Aged, 80 and over; Middle Aged; Female; Male; Neoplastic Cells, Circulatingen
dc.titleDetection of circulating tumor cells in colorectal cancer by Immunobead-PCR is a sensitive prognostic marker for relapse of diseaseen
dc.typeJournal articleen
dc.identifier.rmid0030004074en
dc.identifier.doi10.1007/BF03401893en
dc.identifier.pubid68080-
pubs.library.collectionPhysiology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidHardingham, J. [0000-0001-8277-1199]en
Appears in Collections:Physiology publications

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