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https://hdl.handle.net/2440/122269
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Type: | Journal article |
Title: | Capacitation IVM improves cumulus function and oocyte quality in minimally stimulated mice |
Author: | Zhao, Y. Liao, X. Krysta, A.E. Bertoldo, M.J. Richani, D. Gilchrist, R.B. |
Citation: | Journal of Assisted Reproduction and Genetics, 2020; 37(1):77-88 |
Publisher: | Springer |
Issue Date: | 2020 |
ISSN: | 1058-0468 1573-7330 |
Statement of Responsibility: | Y. ZhaoX. LiaoA.E. KrystaM.J. BertoldoD. RichaniR.B. Gilchrist |
Abstract: | PURPOSE:Oocyte in vitro maturation (IVM) is a patient-friendly reproductive technology but lower success rates than IVF have limited its uptake. Capacitation-IVM (CAPA-IVM) is an innovative new IVM system currently undergoing clinical evaluation. This study aimed to determine temporal effects of the pre-IVM phase of CAPA-IVM on cumulus function and oocyte developmental competence in mildly-stimulated mice. METHODS:Immature cumulus oocyte complexes (COCs) derived from mildly stimulated (23 h PMSG) 28-day-old mice underwent pre-IVM for 0-24 h in medium containing c-type natriuretic peptide (CNP), E2, FSH and insulin, prior to IVM (CAPA-IVM). The effect of pre-IVM duration on cumulus cell function and embryo development post-CAPA-IVM/IVF was assessed. RESULTS:Day 6 blastocyst rate increased incrementally with increasing pre-IVM duration: 40.6 ± 2.0%, 45.8 ± 1.2%, 52.2 ± 3.5%, 53.3 ± 5.9%, and 59.9 ± 2.5% for 0, 2, 6, 12, and 24 h pre-IVM, respectively (P < 0.01). DNA content/COC, a measure of cumulus cell proliferation, was significantly higher with 24 h pre-IVM group compared to 0, 2, or 6 h pre-IVM (P < 0.001). Pre-IVM for 24 h significantly increased cumulus expansion and mRNA expression of matrix genes Has2 and Tnfaip6 and Areg relative to no pre-IVM control (P < 0.01). Cumulus-oocyte gap-junctional communication (GJC) was maintained throughout 24 h pre-IVM (P < 0.0001), and GJC loss was slowed during the subsequent IVM phase, whilst meiotic resumption was accelerated (P < 0.05). Pre-IVM increased COC ATP and ADP content (P < 0.05), but not AMP, ATP/ADP, and energy charge. CONCLUSION:The pre-IVM phase of CAPA-IVM improves the quality of IVM oocytes in a temporally dependent manner and significantly influences cumulus cell function including increased cell proliferation, cumulus expansion, and prolonged cumulus-oocyte GJC. |
Keywords: | Capacitation-IVM (CAPA-IVM) Cumulus-oocyte gap junction communication Oocyte in vitro maturation (IVM) Oocyte quality Pre-maturation (pre-IVM) c-type natriuretic peptide (CNP) |
Description: | Published online: 31 October 2019 |
Rights: | © Springer Science+Business Media, LLC, part of Springer Nature 2019 |
DOI: | 10.1007/s10815-019-01610-x |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1076004 http://purl.org/au-research/grants/nhmrc/1062762 http://purl.org/au-research/grants/nhmrc/1139763 http://purl.org/au-research/grants/nhmrc/1023210 http://purl.org/au-research/grants/nhmrc/1117538 |
Published version: | http://dx.doi.org/10.1007/s10815-019-01610-x |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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