Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/12232
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DC Field | Value | Language |
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dc.contributor.author | Brookes, J. | - |
dc.contributor.author | Geary, S. | - |
dc.contributor.author | Ganf, G. | - |
dc.contributor.author | Burch, M. | - |
dc.date.issued | 2000 | - |
dc.identifier.citation | Marine and Freshwater Research, 2000; 51(8):817-823 | - |
dc.identifier.issn | 1323-1650 | - |
dc.identifier.issn | 1448-6059 | - |
dc.identifier.uri | http://hdl.handle.net/2440/12232 | - |
dc.description | © CSIRO 2000 | - |
dc.description.abstract | This study reports the use of a technique to determine nutrient limitation of cultured and natural phytoplankton. The technique, an FDA-activity assay, which is usually used to assess cell viability, was used to measure metabolic activity in response to nutrient addition; the metabolic activity of phytoplankton was determined as the rate of hydrolysis of fluorescein diacetate (FDA), by intracellular esterases, to fluorescein, which was detectedusing a flow cytometer. Replacement of the limiting nutrient to nitrogen- or phosphorus-limited cultures and field populations resulted in an increase in metabolic activity that was detectable 24 h after nutrient addition. By flow cytometry, the natural phytoplankton community can be divided into different taxonomic groups; the response of these to FDA could be determined individually to allow identification of the nutrients limiting each type of phytoplankton. This would be more specific than the assessment of a whole-community response, which may mask subtle differences among taxa. | - |
dc.language.iso | en | - |
dc.publisher | C S I R O Publishing | - |
dc.source.uri | http://dx.doi.org/10.1071/mf00048 | - |
dc.title | Use of FDA and flow cytometry to assess metabolic activity as an indicator of nutrient status in phytoplankton | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1071/MF00048 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Brookes, J. [0000-0001-8408-9142] | - |
Appears in Collections: | Aurora harvest 7 Earth and Environmental Sciences publications Ecology, Evolution and Landscape Science publications Environment Institute Leaders publications |
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