Characterisation of two arbuscular mycorrhizal fungi in symbiosis with Allium porrum: colonization, plant growth and phosphate uptake

Abstract

<jats:p>Two arbuscular mycorrhizal fungi, <jats:italic>Scutellospora calospora</jats:italic> and <jats:italic>Glomus</jats:italic> sp. ‘City Beach’, were grown in soil conditions suitable for colonization of <jats:italic>Allium porrum</jats:italic>. Effects on plant growth and phosphate uptake were examined. Isolates of <jats:italic>S. calospora</jats:italic> (including the one used here) have been shown by others to vary in their stimulation of plant growth, and appear to be inefficient in the transfer of P from the fungus to the host. Our hypothesis was that such isolates of <jats:italic>S. calospora</jats:italic> may be more aggressive colonizers than other fungi, thus preventing growth increases or producing strong growth depressions in the host plant. In fact, inoculation with either fungus increased growth of plants in their respective soils both low in P (P<jats:sub>0</jats:sub>) and with added P (P<jats:sub>1</jats:sub>) by 42 d. The effect on growth due to mycorrhizal symbiosis (i.e. mycorrhizal growth response, % MGR) at 42 d was higher in plants grown in P<jats:sub>0</jats:sub> soil. Plants colonized by <jats:italic>Glomus</jats:italic> sp. ‘City Beach’ had a greater % MGR than plants colonized by <jats:italic>S. calospora</jats:italic>. Both fungi colonized plants to high levels. The percentage of root length colonized was higher in P<jats:sub>0</jats:sub> soil than in P<jats:sub>1</jats:sub> soil at 21 d. The internal development of <jats:italic>S. calospora</jats:italic> appeared less affected by addition of P than <jats:italic>Glomus</jats:italic> sp. ‘City Beach’ at the early harvests. Formation of arbuscules followed the same trends as total colonization. Shoot P concentration was significantly higher in mycorrhizal plants than in non‐mycorrhizal plants, by 21 d in P<jats:sub>1</jats:sub> soil and by 28 d in P<jats:sub>0</jats:sub> soil. Different percentage responses to added P based on total plant dry weight (% PGR) were observed at 28 and 42 d between the plants colonized by the two fungi. The increased P content due to mycorrhizal colonization (% MPR) differed with soil P. For both fungi grown in their respective soils, the response was greater in plants grown in P<jats:sub>0</jats:sub> soil. Although the isolate of <jats:italic>S. calospora</jats:italic> used is an aggressive arbuscular mycorrhizal fungus with some hosts, it promotes a strong positive plant growth response in <jats:italic>A. porrum</jats:italic> after a mild initial growth depression.</jats:p>