Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/123124
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Type: | Journal article |
Title: | A vasoactive role for endogenous relaxin in mesenteric arteries of male mice |
Author: | Leo, C.H. Jelinic, M. Gooi, J.H. Tare, M. Parry, L.J. |
Citation: | PLoS One, 2014; 9(9):e107382-1-e107382-12 |
Publisher: | Public Library Science |
Issue Date: | 2014 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Bolego, C. |
Statement of Responsibility: | Chen Huei Leo, Maria Jelinic, Jon H. Gooi, Marianne Tare, Laura J. Parry |
Abstract: | The peptide hormone relaxin has striking effects on the vascular system. Specifically, endogenous relaxin treatment reduces myogenic reactivity through nitric oxide (NO)-mediated vasorelaxation and increases arterial compliance in small resistance arteries. However, less is known about the vascular roles of endogenous relaxin, particularly in males. Therefore, we used male wild-type (Rln+/+) and relaxin knockout (Rln-/-) mice to test the hypothesis that passive wall properties and vascular reactivity in mesenteric arteries would be compromised in Rln-/- mice. Passive compliance was determined in arteries (n=8-9) mounted on a pressure myograph and in Ca2+-free Krebs containing 2 mM EGTA. Passive volume compliance was significantly (P=0.01) decreased in the mesenteric arteries of Rln-/- mice. Vascular reactivity was assessed using wire myography. In mesenteric arteries (n=5) of Rln-/- mice, there was a significant (P<0.03) increase in sensitivity to the vasoconstrictors phenylephrine and thromboxane-mimetic U41669. This enhanced responsiveness to vasoconstrictors was abolished by endothelial denudation, and attributed to impaired NO and prostanoid pathways in Rln-/- mice. Sensitivity to the endothelial agonist acetylcholine was significantly (n=7-9, P ≤ 0.03) decreased, and this was abolished in the presence of the cyclooxygenase inhibitor, indomethacin (2 µM). This indicates that prostanoid vasoconstrictor pathways were upregulated in the mesenteric arteries of Rln-/- mice. In summary, we demonstrate endothelial dysfunction and impaired arterial wall remodeling in male mice deficient in relaxin. Thus, our results highlight a role for endogenous relaxin in the maintenance of normal mesenteric artery structure and function in males. |
Keywords: | Mesenteric Arteries Endothelium, Vascular Animals Mice, Knockout Mice Phenylephrine Relaxin Vasoconstrictor Agents Vasoconstriction Vasodilation Male |
Rights: | © 2014 Leo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0107382 |
Grant ID: | http://purl.org/au-research/grants/arc/LP110200543 |
Published version: | http://dx.doi.org/10.1371/journal.pone.0107382 |
Appears in Collections: | Aurora harvest 8 Environment Institute publications |
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hdl_123124.pdf | Published version | 2.04 MB | Adobe PDF | View/Open |
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