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https://hdl.handle.net/2440/124960
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Type: | Journal article |
Title: | Hypoxia-mimicking mesoporous bioactive glass scaffolds with controllable cobalt ion release for bone tissue engineering |
Author: | Wu, C. Zhou, Y. Fan, W. Han, P. Chang, J. Yuen, J. Zhang, M. Xiao, Y. |
Citation: | Biomaterials, 2012; 33(7):2076-2085 |
Publisher: | Elsevier |
Issue Date: | 2012 |
ISSN: | 0142-9612 1878-5905 |
Statement of Responsibility: | Chengtie Wua, Yinghong Zhou, Wei Fan, Pingping Han, Jiang Chang, Jones Yuen ... et al. |
Abstract: | Low oxygen pressure (hypoxia) plays an important role in stimulating angiogenesis; there are, however, few studies to prepare hypoxia-mimicking tissue engineering scaffolds. Mesoporous bioactive glass (MBG) has been developed as scaffolds with excellent osteogenic properties for bone regeneration. Ionic cobalt (Co) is established as a chemical inducer of hypoxia-inducible factor (HIF)-1α, which induces hypoxia-like response. The aim of this study was to develop hypoxia-mimicking MBG scaffolds by incorporating ionic Co2+ into MBG scaffolds and investigate if the addition of Co2+ ions would induce a cellular hypoxic response in such a tissue engineering scaffold system. The composition, microstructure and mesopore properties (specific surface area, nano-pore volume and nano-pore distribution) of Co-containing MBG (Co-MBG) scaffolds were characterized and the cellular effects of Co on the proliferation, differentiation, vascular endothelial growth factor (VEGF) secretion, HIF-1α expression and bone-related gene expression of human bone marrow stromal cells (BMSCs) in MBG scaffolds were systematically investigated. The results showed that low amounts of Co (<5%) incorporated into MBG scaffolds had no significant cytotoxicity and that their incorporation significantly enhanced VEGF protein secretion, HIF-1α expression, and bone-related gene expression in BMSCs, and also that the Co-MBG scaffolds support BMSC attachment and proliferation. The scaffolds maintain a well-ordered mesopore channel structure and high specific surface area and have the capacity to efficiently deliver antibiotics drugs; in fact, the sustained released of ampicillin by Co-MBG scaffolds gives them excellent anti-bacterial properties. Our results indicate that incorporating cobalt ions into MBG scaffolds is a viable option for preparing hypoxia-mimicking tissue engineering scaffolds and significantly enhanced hypoxia function. The hypoxia-mimicking MBG scaffolds have great potential for bone tissue engineering applications by combining enhanced angiogenesis with already existing osteogenic properties. |
Keywords: | Hypoxia; mesoporous bioactive glass; bone tissue engineering; VEGF secretion; HIF-1α expression |
Rights: | © 2011 Elsevier Ltd. All rights reserved. |
DOI: | 10.1016/j.biomaterials.2011.11.042 |
Grant ID: | http://purl.org/au-research/grants/arc/DP120103697 |
Published version: | http://dx.doi.org/10.1016/j.biomaterials.2011.11.042 |
Appears in Collections: | Aurora harvest 8 Biochemistry publications |
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