Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/13000
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Type: Journal article
Title: Molecular analysis of an interspecific hybrid ornamental eucalypt for parental identification
Author: Delaporte, K.
Collins, G.
Conran, J.
Sedgley, M.
Citation: Euphytica, 2001; 122(1):165-170
Publisher: Kluwer Academic Publ
Issue Date: 2001
ISSN: 0014-2336
1573-5060
Statement of
Responsibility: 
K.L. Delaporte, G.G. Collins, J.G. Conran & M. Sedgley
Abstract: The parentage of ‘Urrbrae Gem’, an ornamental hybrid Eucalyptus, was investigated using RAPD-PCR. The female parent was known to be E. erythronema var. erythronema but previous opinion, based on adult morphological characters, placed the male parent as either E. stricklandii or E. gomphocephala. Samples of DNA, extracted from leaves of different individuals within each species, were amplified with six different 10-mer primers to produce RAPD fingerprints. These were used to generate a UPGMA dendrogram based on genetic similarities, and an ordination plot, derived by multi-dimensional scaling (MDS), and minimum spanning tree (MST) to show the relative dissimilarities between the individuals tested. The UPGMA dendrogram divided the samples into three clusters, with the hybrid slightly closer to E. stricklandii than E. gomphocephala. The MDS ordination and MST placed the hybrid between E. erythronema var. erythronema and E. stricklandii, supporting E. stricklandii as the male parent.
Keywords: Eucalyptus; hybrid paternity; multivariate analysis; RAPD-PCR; UPGMA
Description: The original publication can be found at www.springerlink.com
RMID: 0020010079
DOI: 10.1023/A:1012619314419
Appears in Collections:Wine Science publications
Environment Institute publications

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