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https://hdl.handle.net/2440/130461
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dc.contributor.author | Nguyen, H.T. | - |
dc.contributor.author | O’Donovan, L. | - |
dc.contributor.author | Venter, H. | - |
dc.contributor.author | Russell, C.C. | - |
dc.contributor.author | McCluskey, A. | - |
dc.contributor.author | Page, S.W. | - |
dc.contributor.author | Trott, D.J. | - |
dc.contributor.author | Ogunniyi, A.D. | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Antibiotics, 2021; 10(3):307-307 | - |
dc.identifier.issn | 2079-6382 | - |
dc.identifier.issn | 2079-6382 | - |
dc.identifier.uri | http://hdl.handle.net/2440/130461 | - |
dc.description.abstract | In this study, we optimized and compared different transmission electron microscopy (TEM) methods to visualize changes to Gram-negative bacterial morphology induced by treatment with a robenidine analogue (NCL195) and colistin combination. Aldehyde-fixed bacterial cells (untreated, treated with colistin or NCL195 + colistin) were prepared using conventional TEM methods and compared with ultrathin Tokuyasu cryo-sections. The results of this study indicate superiority of ultrathin cryo-sections in visualizing the membrane ultrastructure of Escherichia coli and Pseudomonas aeruginosa, with a clear delineation of the outer and inner membrane as well as the peptidoglycan layer. We suggest that the use of ultrathin cryo-sectioning can be used to better visualize and understand drug interaction mechanisms on the bacterial cell membrane. | - |
dc.description.statementofresponsibility | Hang Thi Nguyen, Lisa A. O’Donovan, Henrietta Venter, Cecilia C. Russell, Adam McCluskey, Stephen W. Page ... et al. | - |
dc.language.iso | en | - |
dc.publisher | MDPI AG | - |
dc.rights | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | - |
dc.source.uri | http://dx.doi.org/10.3390/antibiotics10030307 | - |
dc.subject | Transmission electron microscopy; bacterial cell wall; bacterial membrane; Gram-negative bacteria; colistin; drug interaction; Tokuyasu cryo-ultramicrotomy | - |
dc.title | Comparison of two transmission electron microscopy methods to visualize drug-induced alterations of gram-negative bacterial morphology | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.3390/antibiotics10030307 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/LP110200770 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Trott, D.J. [0000-0002-8297-5770] | - |
dc.identifier.orcid | Ogunniyi, A.D. [0000-0001-9308-5629] | - |
Appears in Collections: | Aurora harvest 8 Pharmacology publications |
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