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|Title:||Regeneration of almond from immature seed cotyledons|
|Citation:||Plant Cell Tissue and Organ Culture, 2001; 67(3):221-226|
|Publisher:||Kluwer Academic Publ|
|Phillip J. Ainsley, Freddi A. Hammerschlag, Terry Bertozzi, Graham G. Collins and Margaret Sedgley|
|Abstract:||Adventitious shoots were regenerated from immature cotyledons of four almond cultivars (`Ne Plus Ultra', `Nonpareil', `Carmel' and `Parkinson'). Open-pollinated fruit were collected from orchard-grown trees 100–115 days after full bloom. The proximal ends of the cotyledons were excised and the embryonic axes discarded. The effects of different concentrations of thidiazuron (TDZ) and indole-3-butyric acid (IBA) and the presence or absence of light for the first 7 days of culture were determined. Shoot regeneration rates were highest for cotyledons cultured for 8 weeks on Murashige and Skoog (MS) basal medium containing TDZ (10.0 mgrM), followed by 4 weeks on medium without plant growth regulators. Regeneration levels were further improved if cotyledons were maintained in darkness for the first 7 days. IBA (0.5 mgrM) significantly reduced the development of adventitious shoots. The frequency of cotyledons that developed adventitious shoots under the optimum tested conditions for `Ne Plus Ultra', `Nonpareil', `Carmel', and `Parkinson' were 80.0%, 73.3%, 100.0% and 86.7%, respectively.|
|Keywords:||adventitious shoots; indole-3-butyric acid; organogenesis; Prunus dulcis Mill; thidiazuron|
|Description:||The original publication can be found at www.springerlink.com|
|Rights:||© 2001 Kluwer Academic Publishers|
|Appears in Collections:||Wine Science publications|
Environment Institute publications
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